Preventive Nutrition and Food Science
- 제6권1호
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- Pages.79-81
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- 2001
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- 2287-1098(pISSN)
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- 2287-8602(eISSN)
Protein Engineering of Deoxynucleoside Kinase from Lactobacillus acidophilus: Effect of Site-Directed Mutagenesis on Microbial Growth
- Park, Inshik (Faculty of Food Science, Dong-A University) ;
- Kim, Eun-Ae (Faculty of Food Science, Dong-A University) ;
- Bang, Keuk-Seung (Faculty of Food Science, Dong-A University) ;
- Kim, Seok-Hwan (Faculty of Food Science, Dong-A University) ;
- Kim, Gi-Nahm (Faculty of Food Science, Dong-A University) ;
- Lee, Min-Kyung (Faculty of Food Science, Dong-A University) ;
- Kil, Ji-Oeun (Faculty of Food Science, Dong-A University)
- 발행 : 2001.03.01
초록
Deoxynucleoside kinases exist as heterodimeric pairs specific for deoxyadenosine/deoxyguanosine kinase (dAK/dGK) and deoxyadenosine/deoxycytidine kinase (dAK/dCK). The aspartic acid-84 in dGK was mutated to alanine, asparagine and glutamic acid by site-directed mutagenesis. The mutation resulted in a drastic decease in dGK activity compared to the unmodified cloned enzyme while it increased production of dAK activity. The mutated dak/dgk genes, which synthesize tandem deoxyadenosine/deoxyguanosine kinase, were inserted back to the Lactobacillus acidophilus and Lactococcus lactis by electroporation to determine the effect of site-directed mutation of he enzymes on the microbial growth. However, no significant change was observed in cell growth and lactic acid production between wild type and mutant lactic acid bacteria.