Journal of Acupuncture Research

Korean Acupuncture & Moxibustion Medicine Society (대한침구의학회)
권호 표지

Beneficial Effect of Scutellaria Balicalensis Georgi Extract ont-Buthylhydroperoxide-Induced Inhibition of Organic Cation in Rabbit Renal Cortical Slices

황금약침액(黃芩藥鍼液)이 토끼의 신장절편에서 t-BHP로 유발된 유기양이온의 이동장애에 미치는 영향(影響)

  • Jo, Mee-hyeong (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Dong-Eui University) ;
  • Jang, Kyung-jeon (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Dong-Eui University)
  • 조미형 (동의대학교 한의과대학 침구경혈학교실) ;
  • 장경전 (동의대학교 한의과대학 침구경혈학교실)
  • Received : 2001.07.06
  • Accepted : 2001.07.21
  • Published : 2001.08.20
Download PDF
⟨ Previous Next ⟩

Abstract

Objective : This study was undertaken to determine whether Scutellaria balicalensis Georgi (SbG) extract exerts the protective effect against oxidant-induced alterations in organic cation transport in the renal proximal tubule. Methods : Organic cation transport was estimated by examining alterations in tetraethylammon - ium(TEA) uptake in rabbit renal cortical slices. The slices were treated with 0.2 mM tBHP for 60 min at $37^{\circ}C$. tBHP caused an inhibition in TEA uptake by renal cortical slices. Such an effect was accompanied by depressed Na+-K+-ATPase activity and ATP depletion. tBHP also induced a significant increase in LDH release. Results : SbG prevented tBHP-induced inhibition of TEA uptake in a dose-dependent manner at the concentration ranges of 0.05-0.1%. tBHP-induced inhibition of Na+-K+-ATPase activity and ATP depletion were significantly prevented by 0.05% SbG. tBHP-induced LDH release also was blocked by SbG. tBHP caused a significant increase in lipid peroxidation and its effect was prevented by SbG. Conclusion : These results suggest that SbG prevents oxidant-induced alterations in organic cation transport in rabbit renal cortical slices. Such protective effects of SbG may be attributed to inhibition of peroxidation of membrane lipid.

Keywords

References

  1. 臨床腎系學硏究 杜鎬京
  2. 東醫腎系內科學 杜鎬京
  3. Chem.Pharm.Bull. v.36 Two new flavonoid and other constituents in licorice root: their relative astringency and radical scavenging effects. Hatano. T.;Kagawa. H.;Yasuhara. T.;Okuda. T.
  4. FASEB J. v.4 Role of oxygen free radical in carcinogenesis and brain ischemia. Floyd, R.A.
  5. Am. J. Physiol. v.251 Reactive oxygen species : production and role in the kidney. Baud. L.;Ardaillou. R.
  6. Kidney Int. v.29 A role for oxygen freeradicals in aminonucleoside nephrosis. Diamond, J.R.;Bonventre, J.V.;Karnovsky, M.J.
  7. Curr. Opin. Nephrol. Hypertens. v.2 Postischemic renal injury due to oxygen radicals. Johnson, K.J.;Weinberg, J.M.
  8. 神農本草經輯注 馬繼興(主編)
  9. 原色臨床本草學 辛民敎(編著)
  10. 한의학연구소 논문집 황금의 혈관이완효과에 대한 기전. 金昊顯
  11. 대한면역학회지 v.20 황금 추출물이 면역기능. 미생물증식 및 세균변이에 미치는 영향. 이정호;신숙정;문용
  12. 침구학회지 v.16 no.1 黃芩藥鍼液의 흰쥐 肝細胞內의 抗酸化效能에 관한 연구. 金性逸;都垣錫;金甲成
  13. Anal. Biochem. v.86 Dete-rmination of malonaldehyde precusor in tissue by thiobarbituric acid test. Uchiyama M.;Mihara M.
  14. Anal. Biochem. v.72 A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dry binding. Bradford, M.M.
  15. J. Biol. Chem. v.66 The colorimetric determination of phosphorus Fiske, C.H.;Subbarow. Y.
  16. Acta Pharmacol Toxicol v.31 In vitro studies on the uptake of 14C-labelled tetraethylammonium in mouse kidney. Holm J
  17. 본초학 이상인;안덕균
  18. 한약임상응용 이상인;안덕균;신민교;노승현;이영종;김선희
  19. Planta Med v.43 no.2 Studies on Scutellariae radix Part Ⅱ. : The antibacterial substance. Kubo M.;Kimura Y.;Odani T.;Tani T.;Namba K.
  20. Chem Pharm Bull (Tokyo) v.29 no.9 Studies on Scutellariae Radix. IV. Effects on lipid peroxidation in rat liver. Kimuya Y.;Kubo M.;Tani T.;Arichi S.;Okuda H.
  21. Free Radicals--in Biology. Antioxidant aging and longevity. Cutler, R.G.
  22. The free radical theory of aging. free radical reaction in medicine. Feher J.;Csomos G.;Verekei. A.
  23. Nihon Jagkuan Tokyo. SOD and active oxygen moduiater. Oyanagui. Y.
  24. FASEB J. v.9 Oxidative processes and antioxidative defense mechanism in the againg brain. Reiter, R.J.
  25. J Lab Clin-Med. v.119 Free radicals. antioxidants and human disease. Where are we now? Halliwell B;Gutteridge, J.M.C.;Cross, C.E.
  26. Mereck Index twelfth edition. Susan Budavari.
  27. Am J Physiol. v.255 Mechansims of t-butylhydroperoxide-induced toxicity to rabbit renal proximal tubules. Schnellmann, R.G.
  28. Annual Review of pharmacology v.12 the pharmacology of mercury compounds Clarkson, T.W.;Elliott, H.W.;Okun R.;George. R.
  29. 생리학 黃澍寬;全世烈
  30. 東國論集 自然科學編 v.15 紫胡가 freeradical에 의한 지질과산화물 생성에 미치는 영향. 문진영;최미정;남경수;임종국
  31. 生理學 李炳熙
  32. J Pharmacol Exp Ther. v.268 Regulation by protein kinase C of the contraluminal transport system for organic cations in rabbit kidney S2Proximal tubules. Hohage H.;Morth, D.M.;Querl, I.U.;Grefen J