The Journal of Internal Korean Medicine (대한한방내과학회지)

The Society of Internal Korean Medicine (대한한방내과학회)
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The Effect of Artemisia Capillaris Herba on Ethanol-Induced Cytokines(TNF-${\alpha},IL-1{\alpha}$) Secretion in Hep G2 Cells

인진호가 Hep G2 세포에서 에탄올 매개성 Cytokine 분비에 미치는 영향

  • Sim, Jung-Sub (Department of Internal Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Kim, Il-Hwan (Department of Internal Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Kim, Gang-San (Department of Internal Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Kagn, Byung-Ki (Department of Internal Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Choi, Su-Deock (Department of Internal Medicine, College of Oriental Medicine, Wonkwang University)
  • 심정섭 (원광대학교 한의과대학 간계내과학교실) ;
  • 김일환 (원광대학교 한의과대학 간계내과학교실) ;
  • 김강산 (원광대학교 한의과대학 간계내과학교실) ;
  • 강병기 (원광대학교 한의과대학 간계내과학교실) ;
  • 최수덕 (원광대학교 한의과대학 간계내과학교실)
  • Published : 2001.03.30
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Abstract

A human hepatoma cell line, Hep G2 cells, is reliable for the study of alcohol-induced hepatotoxicity. The aim of this study is to determine the relationship between TNF-${\alpha}$, IL-$1{\alpha}$ production and EtOH-induced cytotoxicity on Hep G2 cells. The cells were incubated with EtOH in the presence of Artemisia Capillaris Herba(AC) for 24 hours and in the absence of AC for 48 hours. Cytoviability and cytokines release were analyzed by MTT assay and enzyme linked immunosorbent assay (ELISA), respectively. After 24 hours of EtOH exposure, the cytoviability had markedly decreased, and the release of cytokines had increased. The increased amount of cytokines contributed to EtOH-induced cytotoxicity. Anti-TNF-${\alpha}$ and IL-$1{\alpha}$ antibodies almost abolished it. Interestingly, EtOH-induced cytotoxicity and cytokines production were inhibited by AC. Moreover, when AC was used in combination with antibodies, there was a marked inhibition of EtOH-induced cytotoxicity. These results suggest that EtOH-induced cytotoxicity may regulate, by various factors, and AC may prevent the cytotoxicity through partial inhibition of the $TNF-{\alpha}$ and IL-$1{\alpha}$ secretion.

Keywords

References

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