The Journal of Internal Korean Medicine (대한한방내과학회지)

The Society of Internal Korean Medicine (대한한방내과학회)
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The Effect of Hepatitis Treatment-Tang No.1 on Ethanol-Induced Cytotoxicity of Hep G2 Cells

Hep G2 세포에서 간염제1탕의 에탄올에 의한 세포독성 억제효과

  • Park, Young-Kweon (Department of Internal Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Kim, Gang-San (Department of Internal Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Kang, Byung-Ki (Department of Internal Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Ra, Ki-Ung (Department of Internal Medicine, College of Oriental Medicine, Wonkwang University)
  • 박용권 (원광대학교 한의과대학 간계내과학교실) ;
  • 김강산 (원광대학교 한의과대학 간계내과학교실) ;
  • 강병기 (원광대학교 한의과대학 간계내과학교실) ;
  • 나기웅 (원광대학교 한의과대학 간계내과학교실)
  • Published : 2001.03.30
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Abstract

Object : Hepatitis Treatment-tang No.1 has been used for the treatment of Liver disease and Jaundice. Long-term EtOH exposure leads to immunoregulatory and detoxification impairment. This study aimed to determine the relationship between TNF-${\alpha}$ production and expression, and EtOH-induced cytotoxicity on Hep G2 cells. Method : Cells were incubated with EtOH in the presence or absence of HT. The cells were tested after 24 hours and, again, after 48 hours. Cytoviability and TNF-${\alpha}$ release were analyzed by MTT assay and enzyme linked immunosorbent assay (ELISA), respectively. After 24 hours of EtOH exposure, the cytoviability decreased, and the release of TNF-${\alpha}$ was increased. Increased amounts of TNF-${\alpha}$ contribute to EtOH-induced cytotoxicity. The Anti-TNF-${\alpha}$ antibody almost abolished it. Interestingly, EtOH-induced cytotoxicity and TNF-${\alpha}$ production were inhibited by HT. Moreover, when HT was used in combination with the anti-TNF-${\alpha}$ antibody, there was a marked inhibition of EtOH-induced cytotoxicity. Results : These results suggest that HT may prevent the cytotoxicity through partial inhibition of the TNF-${\alpha}$ secretion.

Keywords

References

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