Effect of Whalakyuoleyng-dan plus Yinsamyangwui-tang on Anti-angionesis

활락효영단합인삼양위탕(活絡效靈丹合人蔘養胃湯)이 혈관신생(血管新生) 억제(抑制)에 미치는 영향(影響)

  • Ko, Ki-Wan (Dept. of Oriental Pathology, College of Oriental Medicine, Kyung Hee University) ;
  • Park, Joon-Hyuk (Dept. of Oriental Pathology, College of Oriental Medicine, Kyung Hee University) ;
  • Kang, Hee (Dept. of Oriental Pathology, College of Oriental Medicine, Kyung Hee University) ;
  • Kim, Sung-Hoon (Dept. of Oriental Pathology, College of Oriental Medicine, Kyung Hee University) ;
  • Yu, Young-Beob (Dept. of Oriental Pathology, College of Oriental Medicine, Kyung Hee University) ;
  • Shim, Bum-Sang (Dept. of Oriental Pathology, College of Oriental Medicine, Kyung Hee University) ;
  • Choi, Seung-Hoon (Dept. of Oriental Pathology, College of Oriental Medicine, Kyung Hee University) ;
  • Ahn, Koo-Seok (Dept. of Oriental Pathology, College of Oriental Medicine, Kyung Hee University)
  • Published : 2001.12.30

Abstract

Anti-angiogenesis is one of therapies which have been high-lightened on the research of cancer treatment. Anti-angiogenesis means that new blood vessels are created from a existing capillary tube and it is a important process on metastasis and permeation when cancer is created or formed. Since angiogenesis have been under research, a complete recovery oriented treatment against cancer have been suggested blocking metastasis, delaying the growth of cancer cell, and blocking the supply of oxygen and nutritive substance through the web of blood vessels. Until now, there are several anti-angiogenesis, which have been known to the public, such as thalidomide, angiostatin, endostatin, 2-methoxyestradiol, TNP-470, and marimastat, etc. Additionally, 17 clinical testing projects about anti-angiogenesis are on the process in NCI(National Cancer Institute). Especially, TNP-470 showed effectiveness against cancer on clinical testing after finishing animal testing. Based on existing researches showing that Yinsamyangwui-tang is effective to strengthening body resistance and Whallakhyolenyng-dan effects cells on the inside of blood vessel because Whallakhyolenyng- dan restrains cell adhesion during the restraining period of a blood vessel, I tried to research the effect of Whalakhyolenyng-dan plus Yinsamyangwui-tang on angiogenesis. I made a conclusion putting into operation through using SK-Hep-1 (KCLB 30052), A549(KCLB 10185), AGS(KCLB 21739), and BCE(Bovine Capillary Endothelial Cell). Followings are the results of my experimental research: 1. According to the researching results of anti-cancer activation against cancer cell, Whallkhyoleyng dan plus Yinsamyangwui-tang decreased the number of cancer cells -- While injecting $600{\mu}g/ml$, injected groups decreased 3.1% more comparing with the contrastive group of SK-Hep-1, 49.7% more comparing with the contrastive group of A549, and 31.0% more comparing with the contrastive group of AGS. 2. According to the researching results of DNA composition effect between BCE and cancer cell, Whallakhyoleyng-dan plus Yinsamyangwui-tang reduced the rate of SK-Hep-1 synthesis inhibition by 59.1% at $600{\mu}g/ml$ intensity comparing with contrastive group; for A549, 72.6%; for AGS, 6.1%, for BCE, 28.9%. 3. According to the researching results about the effect of BCE cell to angiogenesis, angiogenesis was restrained at $400{\mu}g/ml$ intensity during 18 hours observation. 4. In the case of aortic ring assay, the half level of angiogenesis was reduced comparing with the contrastive group while injecting with $400{\mu}g/ml$ intensity; with $800{\mu}g/ml$, under 10% comparing with contrastive group; and with $1600{\mu}g/ml$, complete restrain. According to the above results, Whallakhyoleyng-dan plus Yinsamyangwui-tang was proved to have an anti-angiogenetic effects.

Keywords

References

  1. 경희대학교 대학원 석사학위논문 沒藥散이 혈관신생억제에 미치는 효과에 대한 연구 姜大寅
  2. 경희대학교 대학원 석사학위논문 桂枝가 Angiogenesis의 억제기전에 미치는 영향 姜玧熙
  3. 경희대학교 대학원 석사학위논문 가미자도환의 angiogenesis억제효과에 관한 실험적 연구 姜顯淑
  4. Endotoxin으로 誘發된 白鼠의 血栓症에 身痛逐瘀湯이 미치는 影響 金東秀
  5. 경희대학교 대학원 석사학위논문 人蔘養胃湯의 면역증강효과에 관한연구 金奉成
  6. 위암 조직의 종양 혈관 신생 정도 및 염기성 섬유모세포 성장 인자 발현과 예후와의 관련성 김영우
  7. 경희대학교 대학원 박사학위논문 活絡效靈丹이 Angiogenesis 억제기전에 미치는 영향 羅琪煥
  8. 血栓症과 打撲性 充血 및 高脂血症에 順氣導痰湯 및 化痰湯이 미치는 影響 朴元煥
  9. 진행성 위암에서 종양 맥관형성이 예후인자로서의 가치를 가지는가? 朴正于
  10. 위암에서 맥관형성 정도와 vascular endothelial growth factor(VEGF)발현에 관한 연구 박찬국
  11. 경희대학교 대학원 석사학위논문 鬱金이 Angiogenesis 억제기전에 미치는 영향 成熙根
  12. 경희대학교 대학원 박사학위논문 活絡效靈丹이 암전이 억제에 미치는 영향 孫宗坤
  13. 경희대학교 대학원 박사학위논문 부정방암탕이 암전이 억제에 미치는 영향 沈範相
  14. 實驗的 肝瘀血에 미치는 桂枝茯笭丸 및 그 加味方의 效果에 관한 硏究 申鎭湜
  15. 자궁경부암에서 종양의 맥관형성이 암전이에 미치는 영향 申海英
  16. 二陳湯 및 滌痰湯이 Endotoxin으로 誘發된 血栓症에 미치는 影響 吳鐘珉
  17. 中草藥學 上海中醫學院(編)
  18. 中醫臨床手冊 安徽中醫學院
  19. 病理學 中山醫學院
  20. Annual Report on the Cause of Death Statistics(Based on Vital Registration). 18th ed. National Statistical Office
  21. Angiogenesis Inhibitors in Clinical Trials. NCI
  22. Cell v.88 Endostatin; an endogenous inhibitor of angiogenesis and tumor growth O'Reilly, M.S.;Boehm, T.;Shing, Y.;Fukai, N.;Vasios, G.;Lane, W.S.;Flynn, E.;Birkhead, J.R.;01sen, B.R.;Folkman, J.
  23. 암예방 및 치료를 위한 한의학적 연구
  24. 내과학
  25. 東醫寶鑑 許浚
  26. 現代中共의 癌治療 洪元植
  27. 方藥合編 黃道淵
  28. 醫宗損益 黃道淵
  29. 대한한의 학회지 v.5 no.1 韓醫學에서 瘀血에 대한 槪念 康舜沫
  30. 大韓韓醫學會誌 v.8 no.2 Hydrocortisone acetate로 誘發된 瘀血病態model에 關한 硏究 金聖洙
  31. 대한한의학회지 v.19 no.1 한의학계의 암연구동향과 연구 전략에 대한 연구 金聖勳
  32. 제2차 세포주 연구 워크숍 Invasion and angiogenesis assay 김우호
  33. 大韓東醫病理學會誌 v.11 no.1 桃仁承氣湯 및 그 構成藥物이 瘀血病態模型에 美治는 影響 金楨氾;安圭錫;崔昇勳
  34. 대한한방종양학회지 v.4 no.1 한약을 이용한 항암 실험연구의 경향에 관한 고찰 金賢兒;林成祐;李源哲
  35. 경희대학교 대학원 박사학위논문 가미자도환 구성약물의 혈관신생억제효과에 관한 연구 王德仲
  36. 東洋醫學 瘀血槪念의 重要性 有地滋
  37. 東洋醫學 v.2 東醫學의 客觀化와 東西醫學 病名統一을 위한 方法 尹吉榮
  38. Angiogenesis 연구회 창립 기념 심포지엄 Angiogenesis inhibitors-clinical applications 윤성수
  39. 경희대학교 대학원 석사학위논문 十全大補湯이 암전이 억제에 미치는 영향 尹在鎬
  40. 경희대학교 대학원 박사학위논문 立安散이 Angiogenesis 억제기전에 미치는 영향 李紀龍
  41. 경희대학교 대학원 석사학위논문 人蔘養胃湯의 위장관에 미치는 영향 李東炫
  42. 제3의학 v.3 no.1 Angiogenesis의 한의학적 치료전략 이준우;최승훈;안규석
  43. 경희대학교 대학원 석사학위논문 血府逐瘀湯이 癌轉移 抑制기전에 미치는 영향 李眞華
  44. 경희대학교 대학원 석사학위논문 活絡效靈丹이 瘀血病態模型에 미치는 영향 임강민
  45. 경희대학교 대학원 박사학위논문 加味慈挑丸의 항암 및 면역증강효과에 관한 실험적 연구 全英秀
  46. 한국한의학연구원논문집 v.4 한약재의 신생혈관생성 억제 활성에 관한연구 전원경;이태희;윤유식;김연옥;현제
  47. 대전대학교 대학원 박사학위논문 桃紅四物湯加減方의 항암 및 항전이 효과에 관한 연구 趙漢震
  48. 진행성 위암환자에서 종양맥관형성과 예후와의 상관관계 조현근
  49. 대한한방종양학회지 v.1 no.1 한의학의 종양에 대한 인식과 병리론 최승훈
  50. 大田大學校 論文集 v.6 no.2 黃帝內經에서의 瘀血의 認識에 對한 理論的 硏究 崔昇勳
  51. 인체 유방암종에서 matrix metalloproteinase 와 tissue inhibitor of metalloproteinase의 발현과 맥관형성 및 침윤과의 관계 최윤정
  52. 增補 萬病回春 공廷賢
  53. 衛生寶鑑 羅天益
  54. 中醫治癌大成 孟琳升
  55. 醫學綱目 v.39 樓全善
  56. 雲南中醫雜誌 v.6 no.1 血液患者部分血液流變學指標測定 潘可勝
  57. 中華腫瘤治療大成 潘敏求(主編)
  58. 中醫藥抗癌藥 瀋鴻鵠(著)
  59. 腫瘤專輯 北京 當代名醫臨證精華 史宇歷;單書健(主編)
  60. 浙江中醫雜誌 v.2 血瘀的實驗硏究 施永德(外)
  61. 中國藥物學 時逸人
  62. 경희대학교 제1회 동양의학국제심포지움 中國傳統醫學治療癌症的作用和硏究進展 余桂淸
  63. 本草從新 吳儀洛
  64. 六科準繩 王肯堂
  65. 中西醫結合臨床腫瘤內科學 王守章(外)
  66. 醫林改錯 王淸任
  67. 경희대학교 제1회 동양의학 국제심포지움 中醫中藥治療癌症38年的探索 千爾辛
  68. 中醫腫瘤學(上) 郁仁存
  69. 腫瘤硏究 郁仁存;姜延良;于爾辛
  70. 本草綱目 李時珍
  71. 醫宗必讀 李中梓
  72. 癌症的中西醫最新對策 李패文(主編)
  73. 景岳全書 張介賓
  74. 醫學衷中參西錄 張錫純
  75. 活血化瘀硏究與臨床 陳可冀(主編)
  76. 太平惠民和劑局方 v.2 太平惠民和劑局(編)
  77. 中醫雜誌 v.35 no.3 抗癌中藥的生物治療效應硏究近況 邢雪梅;邢聰;邢磊
  78. Rev Med Interns v.19 no.12 Tumoral angiogenesis;physiopathology, prognostic value and therapeutic perspectives Andre, T.;Chastre, E.(et al)
  79. J Am Acad Dermatol v.40 The antiangiogenic agents TNP-470 and 2-methoxyestradiol inhibit the growth of angiosarcoma in mice Arbiser, J.L.(et al)
  80. Pharmac Ther. v.63 Angiogenesis inhibition a review Auerbach, W.;Auerbach , R.
  81. Clin Cancer Res v.5 no.8 A phase l and pharmacokinetic study of TNP-470 administered weekly to patients with advanced cancer Bhargava, P.(et al)
  82. Biochin biophys Acta v.1032 Tumor interactions with the vasculature;angiogenesis and tumor metastasis Blood, C.H.;Zetter, B.R.
  83. J. Natl Cancer Int, v.87 Angio-in bladder cancer Relationship between microvessel density and tumor prognosis, Bochner, B.H.;Cote R.J.;Weidner, N.(et al)
  84. Cell v.85 no.5 Localization of matrix metalloproteinase MMP-2 to the surface of invasive cells by interaction with integrin alpha v beta 3. Brooks, P.C.;Stromblad, S.;Sanders L.C.;VonSchalscha, T.L.;Aimes, R.T.; Stetler-Stevenson, W.G.;Quigley, J.P.;Cheresh, D.A.
  85. J. Med. v.5 no.1 The rational of anticoagulants in the treatment of cancer Cliffton, E.E.;Grossi, C.E.
  86. Proc Natl Acad Sci v.91 no.9 Thalidomide is an inhibitor of angiogenesis. D'Amato, R.J.;Leughanan, M.S.;Flynn, E,;Folkman, J.
  87. Cancer Chemother Pharmacol v.43 Thalidomide up regulates prostate specific antigen secretion from LNCaP cells Dixon, S.C.(et al)
  88. Boll Soc Itai Biol Sper. v.40 no.13 Action of thalidomide on the growth of the chick-embro tibia in organotypic cultures Franceschini, M.(et al)
  89. Cancer J Angiogenesis and cancer metastasis. Fidler, I.F.
  90. J Pharm Sci, v.88 no.1 Pharmacokinetics of thalidomide in an elderly prostate cancer population Figg, W.D.(et al)
  91. Jpathol, v.177 Microvessel count predicts metastatic disease and survival in nom-small cell lung cancer Fontanini, G.;Bigini, D.;Vignati, S.(et al)
  92. Antiangiogenic theraphy In;Cancer: Principles and Practice of Oncology.5th ed. Folkman, J.;Devita V.T.;Helman, S.;Rosenberg, S.A.(eds.)
  93. Nature Medicine. v.1 Angiogenesis in cancer, vascular, rheumatoid, and other disease. Folkman, J.
  94. J Natl Cancer Inst, v.82 What is the evidence that tumors are angiogenesis dependent? Folkman, J.
  95. J. Bio. Chem. v.267 angiogenesis Folkman, J.;Shing, Y.
  96. J clin Oncol v.13 Clinical improtance of the determination of tumor angiogenesis in breast carcinoma:Much more than a new prognostic tool Gasparini, G.;Harris, A.L.
  97. OJ Clin Oncol v.12 Tumor microvessel density, P53 expression, tumor size and peritumoral lymphatic vessel invasion are relevant progostic markers in node-negative breast carcinoma Gasparini, G.;Weindner, N.;Bevilacqua, P.(et al)
  98. N. Y State J. Med. v.15;73 no.2 Malignant tumors with superior vena cava obstruction Ghosh, B.C.;Cliffton, E.E.
  99. Cell v.86 Patterns and emerging mechanism of the angiogenic switch during tumorigenesis Hanahan, D.;Folkman, J.
  100. Lancet. v.349 Antiangiogenesis for cancer therapy Harris, A.L.
  101. Lancet, v.340 Angiogenesis assessed by platelet, endthelial cell adhesion molecule antibodies, as indicator of node metastases and survivai in breast cancer Horak, E.R.;Leek, R.;Klenk, N.(et al)
  102. J Urol v.154 Tumor angiogenesis correlates with Iymphnode metastases in invasive bladder cancer Jaeger, T.M.;Weidner, N.;Chew, K.(et al)
  103. Cancer J Sci Am. v.4 New developments in angiogenesis a major mechanism for tumor growth and target for therapy Jones, A.;Harris, A.L.
  104. Clin Cancer Res, v.3 no.9 A phase I study of TNP-470 administered to patients with advanced squamous cell cancer of the cervix Kudelka, A.P.(et al)
  105. Cancer Treat Res, v.40 Tumor invasion and metastasis;biochemicall mechaninsrils, Liotta, L.A.;Stracke, M.L.
  106. J Clin Oncol v.13 Tumor angiogenesis as a predictor of recurrence in gastric carcinoma Maeda, K.;Chung, Y.S.;Takatsuka, S.(et al)
  107. Surg Clin North Am Aug v.54 no.4 Radiologically occult lung cancer. Report of 26 cases, Martini, N.;Beattie, E.J. Jr.;Cliffton, E.E.;Melamed, M.R.
  108. J Med, v.5 no.1 The incidence and course of Michales, L.
  109. A Novel angiogenesis Inhibitor That Mediates the Suppression of Metastases by a Lewis Lung Carcinoma cell v.79 Michael, S.;0'Reilly;Lars Holmgren;YuenShing;Catherine Chen;Rosalind A;Rosenthal, Marsha Moses;William S.;Lane, Yihai Cao;E. Helene Sage;Judah Folkman Angiostain
  110. A Novel angiogenesis Inhibitor That Mediates the Suppression of Metastases by a Lewis Lung Carcinoma cell v.79 Michael. S.;0'Reilly, Lars Holmgren;Yuen Shing;Catherine Chen;Rosalind A. Rosenthal;Marsha Moses;William S. Lane;Yihai Cao, E.;Helene Sage;Judah Folkman Angiostain
  111. J Immunol Method v.65 no.1;2 T Rapid colorimetric assay for cellular growth and survival application to proliferation and cytotoxicity assays. Mosmann
  112. Tumori v.85 no.1 Regression of metastatic breast cancer in a patient treated with the anti-angiogenlc drug TNP-470 Offodile, R.(et al)
  113. J Cell Physiol v.156 no.2 Type Ⅳ collagenase and TIMPs modulate endothelial cell morphogenesis in vitro. Schnaper, H.W.;Grant, D.S.;Stetler-Stevenson, W.G.;Fridman, R.D;Orazi, Murphy AN;Bird, R.E.;Hoythya, M.;Fuerst, T.R.;French, D.L.(et al)
  114. Am J Pathol v.152 no.2 Activation of coagulation and angiogenesis in cancer, immuno histochemical localization in situ of clotting proteins and vascular endothelial growth factor in human cancer Shoji, M.;Hnacock, W.W.;Abe, K.;Micko, C.;Casper, K.A.;Baine, R.M.;Wilcox, F.X.;Danave, 1.;Dillehy, D.L.;Matthews, E.;Contrino, J.;Morrissey, J.H.;Cordon, S.;Edgington, T.S.;Kudryk, B.;Kreutzer, D.L.;Rickles, F.R.
  115. Am J Obstet Gynecol v.159 The significance of atypical vessels and neovascularization in cervical neoplasia Sillman, F.;Boyce, J.;Fruchter, R.
  116. Eur J Surg Suppl v.582 The diagnostic and prognostic value of tumor angiogenesis Szabo, S.;Sander, Z.
  117. Gynecol Oncol v.56 Tumor angiogenesis as a prognostic factor in cervical carcinoma Wiggins, D.L.;Granai, C.O.;Steinhoff, M.M.(et al)
  118. J Biol Chem v.264 Sv40-transformed human lung fibroblasts secrete a 92-kDa type Ⅳ collagenase which is identical to that secreted by normal human macrophages. Wilhem, S.M.;Collier, I.E.;Harmer, B.L.;Eisen, A.Z.;Grant, G.A.;Goldberg, G.I.
  119. Breast Cancer Res Treat, v.55 no.2 Synergistic action of apoptosis induced by eicosapentaenoic acid and TNP-470 on human breast cancer cells Yamamoto, D.(et al)
  120. Int J Cancer v.75 no.5 Vaschular endothelial growth factor induces tissue factor and matrix metalloproteinase production in endothelial cells, conversion of prothrombin to thrombin results in progelatinase A activation and cellproliferation. Zucher, S.;Mirza, H.;Conner, C.E.;Lorenz, A.F.;Drews, M.H.;Bahou, W.F.;Jesty, J.