Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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A Comparative Study on the Solvent Extraction and Supercritical Fluid Extration Method of ${\beta}-Ecdysone$ in Achyranthis radix

우슬 중 ${\beta}-Ecdysone$의 초임계추출법(SFE)과 용매추출법의 비교

  • Kim, Mi-Ra (Inst. of Agricultural Sci. and Tech., Faculty of Biol. and Environ. Chemistry, College of Agriculture, Chonnam National University) ;
  • Kim, Myong-Seok (Chollanamdo Provincial Agricultural Technology Institute) ;
  • Shim, Jae-Han (Inst. of Agricultural Sci. and Tech., Faculty of Biol. and Environ. Chemistry, College of Agriculture, Chonnam National University)
  • 김미라 (전남대학교 농과대학 농업과학기술연구소, 생물환경화학) ;
  • 김명석 (전남농업기술원 작물과) ;
  • 심재한 (전남대학교 농과대학 농업과학기술연구소, 생물환경화학)
  • Published : 2001.08.31
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Abstract

Extraction efficiency of ${\beta}-ecdysone$ in Achyranthis radix was investigated using the methods of solvent extraction and supercritical fluid extraction (SFE). The optimum SFE conditions for the analysis of ${\beta}-ecdysone$ in A. radix were 300 atm, $80^{\circ}C$, 100 ml of extraction volume, and 20% ratio of modifier(methanol). As revealed through SFE, detection limit of ${\beta}-ecdysone$ in A. radix was 5 ng and recoveries of ${\beta}-ecdysone$ in A. radix shoots and root were $90.7{\sim}93.5%$ and $77.8{\sim}81.9%$, respectively. Recoveries of ${\beta}-ecdysone$ in A. radix shoots and root through solvent extraction were $76.4{\sim}93.0%$ and $80.6{\sim}93.1%$, respectively. ${\beta}-Ecdysone$ content was determined to be highest at 654.9 ppm in the first year A. radix roots harvested in August.

우슬(Achyranthis radix)중 ${\beta}-ecdysone$의 초임계추출법과 용매추출법의 비교를 위해 초임계유체추출(SFE) 및 용매추출 후 초임계유체크로마토그래피(SFC)에 의한 우슬 중 ${\beta}-ecdysone$ 분석을 실시하였다. SFE에 있어서 추출 최적압력은 300 atm이었으며, 온도는 $80^{\circ}C$, 추출량은 100 ml, 보조제인 methanol 함량비율은 20%였다. 우슬에서 ${\beta}-ecdysone$의 분석시 SFC의 최소검출량은 5 ng이었고, SFE의 회수율은 줄기에서는 $90.7{\sim}93.5%$였으며, 뿌리에서는 $77.8{\sim}81.9%$였다. 용매추출의 회수율은 줄기에서는 $76.4{\sim}93.0%$였고 뿌리에서는 $80.6{\sim}93.1%$였다. 우슬의 생육기별 조사에서 ${\beta}-ecdysone$은 주로 뿌리에서 많이 검출되었고 1년생이면서 8월에 수확한 것이 654.9 ppm으로 가장 많이 함유한 것으로 나타났다.

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