Korean Journal of Medicinal Crop Science (한국약용작물학회지)
- Volume 9 Issue 1
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- Pages.15-20
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- 2001
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- 1225-9306(pISSN)
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- 2288-0186(eISSN)
In vitro Propagation of Trichosanthes kirilowii Max.
조직배양에 의한 하늘타리의 증식
- Hwang, Sung-Jin (Department of Food and Biotechnology, Dongshin University) ;
- Hwang, Baik (Department of Biology & Institute of Basic Science, Chonnam National University)
- Published : 2001.03.31
Abstract
Adventitious shoot buds, without an intervening callus phase, were induced from stem explants of Trichosanthes kirilowii on MS medium supplemented with 2 mg/L BA. The culture medium, carbon source as well as plant growth regulators were found to influence further shoot multiplication and eventual shoot growth. A maximum number of shoots was obtained on a MS medium containing 0.5 mg/L BA and 3% sucrose. Shoot produced in vitro were rooted on half-strength phytagelled 1/2MS medium prior to transfer to green house condition.
하늘타리 (T. kirilowii)의 기내 (in vitro) 대량증식을 위하여 줄기절편을 2 mg/L BA가 첨가된 MS 배지에 치상하였을 때 정단부위가 포함된 줄기절편의 기저부로부터 다수의 부정아가 형성되었다. 부정아는 정상적인 신초로 분화하였으며, 이와같이 형성 된 신초는 0.5 mg/L BA가 첨가된 MS 배지로 옮겼을 때 4주 후 시료당
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