BMB Reports
- 제33권3호
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- Pages.285-288
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- 2000
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- 1976-670X(eISSN)
Cytochrome c and Chloroplast were Used for an Artificial Approach to Confirming the Irreversible Catalysis by Mitochondrial Cytochrome Oxidase
- Song, Ji-Young (Department of Biochemistry, Yeungnam University) ;
- Lee, Jae-Yang (Department of Biochemistry, Yeungnam University) ;
- Lee, Sang-Jik (Department of Biochemistry, Yeungnam University)
- 투고 : 2000.01.28
- 심사 : 2000.03.30
- 발행 : 2000.05.31
초록
Ferricytochrome c was artificially made to receive the aqueous electrons evolved through the influence of illuminated chloroplast. This ferricytochrome c, which was bombarded by electrons, was reduced to ferrocytochrome c by making sure that a certain cytochrome is reduced. This may require an electronic attack that is created by the chloroplast inside the plant cell. The possibility of reversing the oxidation of ferrocytochrome c by cytochrome oxidase was examined using a contrived redox system composed of cytochrome oxidase, ferricytochrome c and chloroplast with illumination. We recognized that the oxidase is unserviceable for the reversibleness in spite of the existence of chloroplast.