Clinical and Experimental Reproductive Medicine
- 제27권4호
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- Pages.339-344
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- 2000
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- 2233-8233(pISSN)
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- 2233-8241(eISSN)
인간정자의 처리에 있어서 Percoll과 Sil-Select 방법의 비교
Comparison between Percoll and Sil-Select Methods on the Human Spermatozoa Treatment
- 문신용 (서울대학교 의과대학 산부인과학교실, 서울대학교 의학연구원 인구의학연구소) ;
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류범용
(서울대학교 의학연구원 인구의학연구소) ;
- 신현아 (서울대학교 의학연구원 인구의학연구소) ;
- 오선경 (서울대학교 의학연구원 인구의학연구소) ;
- 서창석 (서울대학교 의과대학 산부인과학교실, 서울대학교 의학연구원 인구의학연구소) ;
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김석현
(서울대학교 의과대학 산부인과학교실 서울대학교 의학연구원 인구의학연구소) ;
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최영민
(서울대학교 의과대학 산부인과학교실, 서울대학교 의학연구원 인구의학연구소) ;
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김정구
(서울대학교 의과대학 산부인과학교실) ;
- 최규홍 (서울대학교 의학연구원 인구의학연구소, 성균관대학교 의과대학 삼성제일병원 산부인과) ;
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이진용
(서울대학교 의과대학 산부인과학교실)
- Moon, Shin-Yong (Department of Obstetrics and Gynecology, College of Medicine, Seoul National University, Institute of Reproductive Medicine and Population, Medical Research Center, Seoul National University) ;
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Ryu, Buom-Yong
(Institute of Reproductive Medicine and Population, Medical Research Center, Seoul National University) ;
- Shin, Hyun-Ah (Institute of Reproductive Medicine and Population, Medical Research Center, Seoul National University) ;
- Oh, Sun-Kyung (Institute of Reproductive Medicine and Population, Medical Research Center, Seoul National University) ;
- Suh, Chang-Suk (Department of Obstetrics and Gynecology, College of Medicine, Seoul National University, Institute of Reproductive Medicine and Population, Medical Research Center, Seoul National University) ;
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Kim, Seok-Hyun
(Department of Obstetrics and Gynecology, College of Medicine, Seoul National University, Institute of Reproductive Medicine and Population, Medical Research Center, Seoul National University) ;
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Choi, Young-Min
(Department of Obstetrics and Gynecology, College of Medicine, Seoul National University, Institute of Reproductive Medicine and Population, Medical Research Center, Seoul National University) ;
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Kim, Jung-Gu
(Department of Obstetrics and Gynecology, College of Medicine, Seoul National University) ;
- Choi, Kyu-Hong (Institute of Reproductive Medicine and Population, Medical Research Center, Seoul National University, Department of Obstetrics and Gynecology, Samsung Cheil Hospital, Sungkyunkwan University, School of Medicine) ;
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Lee, Jin-Yong
(Department of Obstetrics and Gynecology, College of Medicine, Seoul National University)
- 발행 : 2000.12.30
초록
Objective: To evaluate silane-coated silica particles (Sil-select) as an alternative to polyvinylpyrrolidone-coated particles (Percoll) for gradient separation of spermatozoa, for use in assisted reproduction. Methods: 20 normal semen based on WHO criteria were included in this study. Recovery of motile and morphologically normal spermatozoa after using two-layer Percoll and Sil-select gradient respectively was recorded. Motility, HOST (hypoosmotic swelling test) and the detection of malondialdehyde for LPO (lipid peroxidation) after 24 h of incubation at