한국수산과학회지 (Korean Journal of Fisheries and Aquatic Sciences)
- 제33권3호
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- Pages.198-204
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- 2000
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- 0374-8111(pISSN)
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- 2287-8815(eISSN)
대구고니 단백질의 효소적 가수분해물로부터 항산화성 펩타이드의 분리${\cdot}$ 정제 및 특성
Purification and Characterization of Antioxidative Peptides from Enzymatic Hydrolysate of Cod Teiset Protein
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KIM Se-Kwon
(Department of Chemistry Pukyong National University) ;
- CHOI Yong-Ri (Research Institute of Biolmmunity, Yan Bian Medicine College) ;
-
PARK Pyo-Jam
(Department of Chemistry Pukyong National University) ;
- CHOI Jeoung-Ho (Department of Chemistry Pukyong National University) ;
- MOON Sung-Hoon (Department of Chemistry Pukyong National University)
- 발행 : 2000.05.01
초록
수산가공공장에서 원료어 처리시 대량으로 발생하는 비가식부의 하나인 대구의 고니부분을 효율적으로 이용하기 위하여 단백질을 효소로 가수분해시킨 후 한외여과막을 사용하여 분자량별로 분획하였으며, 이들 가수분해물 중 항산화활성이 뛰어난 펩타이드를 이온교환 크로마토그래피, 겔크로마토그래피 및 HPLC로 분리
In order to utilize by-products which would normally be discarded in marine processing plants, cod teiset protein was hydrolyzed and antioxidative actiTity of the hydrolysate was investigated. AntioxidatiTe peptide was isolated using ultrafiltration membrane, ion-exchange chromatography on a SP-Sephadex C-25 column, gel filtration on a Sephadex G-15 column, high performance liquid chromatography on an ODS column, and capillary electrophoresis chromatography. Antioxidative activities of the cod teiset hydrolysate were compared with