발생단계에 따른 한국재래산양 췌장에서 pancreatic polypeptide 면역반응세포에 대한 면역조직화학적 연구

Immunohistochemical study of pancreatic poly-peptide-immunoreactive cells in the pancreas of the Korean native goat (Capra hircus) during developmental stages

태아기, 신생아기, 생후 1개월령, 6개월령 및 성체의 한국재래산양 췌장에서 pancreatic poluypeptide(PP) 면역반응세포의 분포 및 출현빈도를 PAP 법에 의해 검색하였다. 외분비부에서는 모든 연령에서 관찰되었으며 췌장세포 사이에 분포하였다. PP 면역반응세포는 신생아가기에서 생후 1개월에 증가한 후 점차 감소하였다. 췌도관에서는 PP 면역반응세포가 생후 1개월부터 관찰되면 그후 점차 감소하였다. 췌도관에서는 PP 면역반응세포가 생후 1개월부터 관찰되면 그후 점차 감소하였다. 이들 면역반응세포는 생후 1개월령 및 6개월령의 췌도관 상피하 결합조직에서 소수 관찰되었다. 그러나 성체에서는 췌도관 상피세포 사이에서 극소수 관찰되었다. 그러나 성체에서는 췌도관 상피세포 사이에서 극소수 관찰되었다. 내분비부(췌장섬)에서 PP 면역반응세포는 신생아기부터 관찰되며, 생후 1개월령부터의 PP면역세포의 분포는 췌장성 주변부에 중등도 또는 소수가 산재하는 것과 다수의 PP 면역반응세포가 췌도 전체 (생후 1개월령)또는 췌도 중앙부(생후 6개월령)에 산재하는 것 등 두가지 유형을 나타내었다. 결론적으로 한국 재래산양의 췌장에 있어서 PP 면역반응세포의 분포와 출현빈도는 발생단계에 따라 상이하였다. 이는 발생단계 동안의 식이성 및 생리학적 조건변화가 이와같은 상이함을 유발하는 요인이 된 것으로 사료된다.

The regional distribution and relative frequency of the pancreatic polypeptide (PP)-immunoreactive cells in the pancreas of the Korean native goat (Capra hircus) were studied by immunohistochemical methods (PAP methods) using specific antisera against to PP during developmental stage, fetus, neonate, 1-month-old, 6-month-old and adult. The different regional distribution and relative frequency of PP-immunoreactive cells in the pancreas of Korean native goat were observed during development. In the exocrine portions, they were detected in the all ages and distributed in the interacinar regions. PP-immunoreactive cells were increased from neonate to 1-month-old but thereafter decreased with developmental stage. In the pancreatic duct, PP-immunoreactive cells were demonstrated from 1-month-old but they were decreased with developmental stages in these regions. These cells were observed in the subepithelial connective tissue of the pancreatic duct with a few frequencies in 1-and 6-month-old. However, they were located in the interepithelial cells of the pancreatic duct in the adult with rare frequency. In the endocrine portion (pancreatic islets), PP-immunoreactive cells were observed from neonate, and the regional distribution of PP-immunoreactive cells from 1-month-old was divided into two patterns : dispersed 1) in the marginal regions of the pancreatic islets with moderate or a few frequencies, and 2) in the whole pancreatic islets (in a case of 1-month-old) or in the central cores (in a case of 6-month-old and adult) with numerous frequency. In conclusion, the different regional distribution and relative frequency of PP-immunoreactive cells in the pancreas of the Korean native goat were observed during development. It is suggested that the changing of feeding habits and physiological conditions during different developmental stages may cause these differences.