Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
권호 표지

Isolation and Partial Characterization of a Polysaccharide with Antithrombin Activity against Blood Coagulation in Manda®, a Fermented Natural Food

  • Kim, Dong Chung (Department of Food Science, Sunchon First College) ;
  • Okuda, Hiromichi (The Second Department of Medical Biochemistry, School of Medicine, Ehime University Ehime) ;
  • Hwang, Woo Ik (Department of Biochemistry, Medical College, Korea University) ;
  • Jung, Jin (School of Agricultural Biotechnology, Seoul National University)
  • Received : 2000.09.06
  • Published : 2000.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

A polysaccharide with antithrombin activity in Manda$^{(R)}& (PAM) was purified via procedures comprising three major steps, i.e. fractional precipitation with ethanol, anion exchange chromatography, and gel permeation chromatography. PAM showed a symmetrical peak on size exclusion HPLC, as assessed by refractive index, and behaved as a single band on cellulose acetate electrophoresis. The average molecular mass was estimated to be 222 kDa by gel filtration. PAM was found to be a sulfated heteropolysaccharide that contains sulfate group (20.5%, w/w) and uronic acid moiety (7.1 %, w/w) in addition to neutral sugar consisting of fucose, xylose, mannose, galactose, and glucose in a molar ratio of 1.00 : 0.35 : 0.28: 0.22 : 0.15. This polysaccharide appeared to inhibit blood coagulation via the intrinsic pathway in a dose-dependent pattern. The clotting of fibrinogen by thrombin was also significantly mitigated by the presence of PAM.

Keywords