Korean Journal of Soil Science and Fertilizer (한국토양비료학회지)

Korean Society of Soil Science and Fertilizer (한국토양비료학회)
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Application of Near Infrared Reflectance Spectroscopy as a Rapid Leaf Analysis Method to Evaluate Nutritional Diagnosis in Apple (Malus Domestica Borkh, Fuji) and grape(Vitis Labrusca, Campbell Early)

영양진단을 위한 신속한 엽분석 방법으로서 근적외분광분석기의 이용

  • Seo, Young-Jin (Kyongbuk Agricultural Technology Administration) ;
  • Park, Man (Department of Agricultural chemistry, kyungpook National University) ;
  • Kim, Chang-Bae (Kyongbuk Agricultural Technology Administration) ;
  • Kim, Jong-Su (Kyongbuk Agricultural Technology Administration) ;
  • Yoon, Jae-Tak (Kyongbuk Agricultural Technology Administration) ;
  • Cho, Rae-Kwang (Department of Agricultural chemistry, kyungpook National University)
  • Published : 2000.08.30
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Abstract

The Near Infrared Reflectance Spectroscopy(NIR) was used to evaluate nutritional diagnosis for rapid leaf analysis method, 177 'Fuji' apple and 130 'Campbell Early' grape leaves were measured by Near Infrared reflectance spectra in the NIR region(1,100~2.500nm). Total nitrogen content was measured by kjelldhal distillation, after salycilic acid-sulfuric acid digestion. An empirical equation to predict total nitrogen content from its spectral signature was developed by adapting the Near Infrared Reflectance Spectroscopy analysis(NIRa) technique and the results were apple-0.965(R). 0.086(SEC), grape-0.926(R), 0.152(SEC). Standard Error of Prediction(SEP) of NIRa for predicting the total nitrogen of apple and grape leaves was 0.360 and 0.210, respectively. It was concluded that Near infrared reflectance spectroscopy analysis is promising method for rapid analysis of apple and grape leaves.

근적외 분광분석기를 이용하여 과수의 영양진단을 위한 신속한 엽분석 수단으로 활용하고자 본 시험을 수행하였다. 사과 '후지'품종 잎 177점, 포도 '캠벨얼리' 품종 잎130점의 시료를 이용하여 1,100~2.500nm까지 근적외영역의 흡수스펙트럼을 측정하였다. 총질소 분석은 살리실산-황산으로 분해후 켈달 증류하여 측정하였다. 근적외 스펙트럼과 총 질소함량과의 상관분석을 이용, 검량식을 개발하였고 화학분석한 결과에 대한 예측식을 구하였다. 사과는 중회귀계수 0.965, 검량식의 표준오차 0.086, 포도는 중회귀계수 0.926, 검량식의 표준오차 0.152이었다. 개발된 검량식을 이용하여 예측식을 작성한 결과는 사과의 경우 0.360, 포도 0.210의 결과를 얻었다. 지역간, 년차간 발생되는 변이가 보정된다면 신속한 엽분석 수단이 될 것으로 판단된다.

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