분석과학 (Analytical Science and Technology)

  • 제13권5호
  • /
  • Pages.666-674
  • /
  • 2000
  • /
  • 1225-0163(pISSN)
  • /
  • 2288-8985(eISSN)
한국분석과학회 (The Korean Society of Analytical Sciences)
권호 표지

기체크로마토그래피/질량분석법을 이용한 생체시료 중 Polybrominated Biphenyls의 분석법

Determination of Polybrominated Biphenyls in Biota Samples Using Gas Chromatography/Mass Spectrometry

  • 홍종기 (기초과학지원연구소 질량분석팀, 정밀구조분석팀) ;
  • 백인걸 (기초과학지원연구소 질량분석팀, 정밀구조분석팀) ;
  • 김협 (기초과학지원연구소 질량분석팀, 정밀구조분석팀) ;
  • 김도균 (기초과학지원연구소 질량분석팀, 정밀구조분석팀) ;
  • 서정주 (기초과학지원연구소 질량분석팀, 정밀구조분석팀) ;
  • 서종복 (기초과학지원연구소 질량분석팀, 정밀구조분석팀) ;
  • 박현미 (한국과학기술연구원, 특성분석센터) ;
  • 이강봉 (한국과학기술연구원, 특성분석센터)
  • Hong, Jongki (Mass Spectrometry Team, Korea Basic Science Institute) ;
  • Baek, In-Girl (Mass Spectrometry Team, Korea Basic Science Institute) ;
  • Kim, Hyub (Mass Spectrometry Team, Korea Basic Science Institute) ;
  • Kim, Do-Gyun (Mass Spectrometry Team, Korea Basic Science Institute) ;
  • Seo, Jung-Joo (Mass Spectrometry Team, Korea Basic Science Institute) ;
  • Seo, Jong-Bok (Mass Spectrometry Team, Korea Basic Science Institute) ;
  • Park, Hyun-Mee (Advanced Analytical Center, Korea Institute of Science and Technology) ;
  • Lee, Kang-Bong (Advanced Analytical Center, Korea Institute of Science and Technology)
  • 투고 : 2000.07.20
  • 발행 : 2000.10.25
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초록

본 연구는 기체크로마토그래피-질량분석법을 이용한 생물시료 중 polybrominated biphenyls(PBBs)의 분석법을 설명하였다. 생물 시료 중 PBB류는 40mL 아세톤과 80mL의 헥산 혼합용매에서 초음파추출기 20분간 교반하여 추출하였다. 추출액 중 존재하는 지질성분은 농축 황산용액으로 분해한 후 PBB류는 Florisil 컬럼으로 정제하였다. PBB류를 정량분석하기 위하여 정제액은 GC/MS-selected ion monitoring 방법에 의해 분석하였다. 본 실험 조건하에서 전반적인 PBB류의 추출율은 77-111%로 나타났다.

The present study describes an analytical method for the determination of polybrominated biphenyls (PBBs) in biota samples by gas chromatography-mass spectrometry (GC/MS). PBBs are extracted twice from 20 g samples with mixture solvent 40mL acetone and 80mL hexane using ultrasonic agitation for 20 min. Lipids in extracts are degraded by concentrated sulfuric acid and then PBBs are purified with Florisil column. The purified extracts are analyzed by GC/MS-selected ion monitoring mode for the quantitation of PBBs in biota sample. The overall recovery yields of PBBs range between 77 and 111% under these experimental conditions.

키워드

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