Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Isolation of a cDNA Encoding a Chloroplast Triosephosphate Isomerase from Strawberry

  • Kim, In-Jung (Department of Biological Sciences, Korea Advanced Institute of Science and Technology) ;
  • Lee, Byung-Hyun (College of Agriculture, Kyungpook National University) ;
  • Jinki Jo (College of Agriculture, Kyungpook National University) ;
  • Chung, Won-Il (Department of Biological Sciences, Korea Advanced Institute of Science and Technology)
  • Published : 2000.09.01
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Abstract

A cDNA clone encoding chloroplast triosephosphate isomerase (TPI-cp) was isolated from strawberry fruit cDNA library. Sequence analyses indicated that the cDNA contains an open reading frame of 314 amino acids (33.5 kDa) composed of a transit peptide (59 amino acids) in amino terminal region and mature protein (255 amino acids). The existence of transit peptide in the deduced amino acid sequence implies that it encodes a chloroplast isoform. The protein sequence is more similar to other plant chloroplast isoforms than cytosolic isoforms. RNA blot analysis indicated that its expression is ubiquitous in examined five tissues, flowers, leaves, petioles, roots and fruits, and shows differential pattern according to fruit ripening. Genomic DNA blot analysis showed that TPI-cp is encoded by multiple genes in strawberry. Through sequence comparison and phylogenetic tree construction, TPI-cp is distinctively grouped into dicot and chloroplast isoforms.

Keywords

References

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