식물조직배양학회지 (Korean Journal of Plant Tissue Culture)

한국식물생명공학회 (The Korean Society of Plant Biotechnology)
권호 표지

야생 흰 제비꽃(Viola patrinii DC.) 배양세포의 생성과정 중 단백질 및 아미노산의 함량변화

Alterations in Protein and Amino acid Contents During Growth on Culture Cells of Wild Viola (Viola patrinii DC.)

  • 정용모 (경남농업기술원 화훼시험장) ;
  • 임현희 (동아대학교 생명자원과학부) ;
  • 조영수 (동아대학교 생명자원과학) ;
  • 정정한 (동아대학교 생명자원과학부) ;
  • 이재헌 (동아대학교 생명자원과학부) ;
  • 서정해 (경남정보대학 환경조경과) ;
  • 권오창 (동아대학교 생명자원과학부)
  • 발행 : 2000.05.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Friable callus와 compact callus를 배양 단계별로 단백질을 정량한 결과 friable callus가 가장 낮았고 배양 0주째부터 단백질 함량이 서서히 증가하여 배양 3주째 최대함량이 되었으며, 4주째부터는 감소하기 시작했다. 전기영동법으로 단백질의 패턴을 조사한 결과 compact callus와 friable callus간에 특징적인 차이를 보이는 단백질 밴드가 관찰되었다. Friable callus는 약 28 KD, 31 KD과 35 KD에서 특이적으로 나타났으며. compact callus에서는 30 KD부근의 밴드에서 많은 변화가 일어났다. 전체 단백질의 구성 아미노산은 배앙 2∼3주에 가장 높은 함량을 보였고, friable callus에서 그 함량이 가장 낮았다.

Proteins in friable and compact calli of Viola patrinii DC. were analysed. The protein contents in friable calli were lower than those in compact calli. In suspension culture, it increased to the maximum after 3 weeks culture from inoculation and decreased after 4 weeks culture. Several strong lavel signals were detected with the SDS-PAGE analysis. The polypeptides of 28, 31 and 35 KD were observed from the friable cell culture, from the compact cell culture strong band of 30 KD was determined, indicating that these polypeptides may be the major protein occurring during their cultures. Changes in amino acid contents during culture of the viola suspension cells were investigated the amino acid contents were greatest between two and three weeks culture of the viola suspension cells.

키워드

참고문헌

  1. Planta v.164 Establishment and naintenance of friable, embryogenic maize callus and the involvement of L-proline Armstrong CL;Green CE
  2. Anal Biochem v.72 A rapid and sensitive method for quantitation of proteins utilizing to principle-dye binding Bradford MM
  3. J.Plant Physiol v.146 Free Amino Acid Composition of Coconyt (Cocos nucifera L.) Calli under Somatic Embryogenesis Induction Conditions. Manaval C.;M. Noirot;L. Versdeil;A. Blattes;C. Huet;F. Grosdemange;J. Buffard-Morel
  4. Plant Cell, Tissue and Organ Culture v.34 Influence of some exogenous amino acid on the production of maize embryogenic callus and on endogenous amino acid content Claparols I.;M.A.Santos ε J.M.Torne
  5. Planta v.165 The procution of callus capable of plant regeneration from immature embryos of numerous Zea mas genotypes Duncan DR;Willinms ME;Zehr BE ε Widholm JM
  6. Maize for Biological Research Plant regeneration in tissue cultures of maze. Green CE;Rhodes CA;Sheridan WF(ed.)
  7. Korean J. Plant Tissue Culture v.19 no.1 Protein Analysis and Structural Aspects during Somatic Embryogenesis in Oenanthe javanica Kim Y.S.;D.Y. Cho;W.Y. Soh
  8. Korean J. Plant Tissue Culture v.20 no.5 Growth and Differentiation of suspension Culture Carrot Cells II. Alteration in Energe Metabolism Activity and Aromatic Amino Acid Content During Somatic Embryogenesis Kim E.S.;M.W. Kim;Y.H.Kang;T.J.Hna
  9. Korean J. Plant Tissue Culture v.19 no.3 Electrofusion of Protoplasts from Wild Viola and Pansy and the Ultrastructure of the Fusion Product Kwon O.C.;C.H. Chung;J.K. Park;T. Sato;T. Taniguchi;E. Maeda
  10. Jpn J. Crop Sci v.61 Ultrastructure of Electrofused Products from Pansy(Violatricolor) Mesophyll and Wild Viola(V. patrinii) petiole callus protoplasts Kwon O.C;C.H. Chung;T. Sato;T. Taniguchi;E. Maeda
  11. Nature v.227 Cleavage of structural protein during the assembly of the head of bacteriopharge T₄ Leammli, U.K.
  12. Korean J. Plant Tissue Culture v.21 no.1 Protein Synthesis during Somatic Embryo Development and Artificial Seed Germination of Apium graveolens L. after Abscisic Acid or Cold Treatment Soh W.Y.;U.P. Yeo;S.S. So;D.Y. Cho
  13. Proc Natl Acad Sci USA v.80 Embryogenic proteins in somatic embryos of carrot Sung ZR;Okimoto R
  14. Plant Cell Reports v.14 Regeneration of plantlets from petiole callus of wild viola (Viola patrinii DC) Tadahiko Sato;Kwon oh-chang;Hiroshi Miyake;Takeshi Taniguchi;Eizo MAEDA
  15. Theor. Appl. Genet v.70 The effect of parental genotype on initiation of embryogenic callus from the elite mazie(Zea mays L.)germplasm Tomes DT;Smith OS