Korean Journal of Plant Tissue Culture (식물조직배양학회지)

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Micropropagation of Bulbs of Lilium longiflorum by Liquid Shaking Culture

액체 진탕배양에 의한 나팔나리(Lilium longiflorum) 소인경구의 대량증식

  • Published : 2000.01.01
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Abstract

Liquid shaking culture was conducted to investigate the proper culture conditions for the micropropagation of high quality lily using bulblets (3 mm in diameter) obtained from small scale culture. The combinations of 9% sucrose and 10 mM nitrogen or 6% sucrose and 20 mM $NH_4NO_3$ were effective on the growth and weight of micro-bulbs. However, the number of new bulbs was the highest when 20 to 40 mM $NH_4NO_3$ and 3% sucrose were added to the MS medium. The total fresh weight was increased effectively in MS medium supplemented with BA 0.2 mg/L alone under $60\;{\mu}mol{\cdot}m^2{\cdot}sec^{-1}$ intensity. Also bulblet weight was increased at $60\;{\mu}mol{\cdot}m^2{\cdot}sec^{-1}$ intensity, regardless of BA concentrations (0.2 and 2 mg/L) in the medium. The proper culture period of bulblet was about 2 month in liquid shaking culture.

백합의 건전 우량주의 확보와 대량 증식을 위해 소인편을 배양하여 얻어진 직경 3mm 정도 크기의 소인경구를 사용하여 액체배양을 실시하였다. 인경구의 생장에 미치는 sucrose와 질소 농도의 영향을 알아본 결과 인경구의 생장과 무게에 가장 효과적인 처리구는 10 mM $NH_4NO_3$에 sucrose 9% 또 는 20 mM $NH_4NO_3$에 sucrose 6%인 것으로 나타났으며, 새로운 소인경구의 분구는 $NH_4NO_3$, 20 또는 40 mM에 sucrose 3%에서 가장 양호하였다. 또한 전체 생체중은 광도 $60\;{\mu}mol{\cdot}m^2{\cdot}sec^{-1}$ BA 0.2 mg/L 단독 처리구에서 효과적이었으며, 구중은 BA에 농도에 큰 차이없이 0.2와 2 mg/L 처리에 광도 $60\;{\mu}mol{\cdot}m^2{\cdot}sec^{-1}$에서 증가하는 경향을 보였다. 액체 진탕배양에 적절한 소인경구의 배양기간은 2개월 정도인 것으로 나타냈다.

Keywords

References

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