미생물학회지 (Korean Journal of Microbiology)

  • 제36권1호
  • /
  • Pages.8-13
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  • 2000
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  • 0440-2413(pISSN)
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  • 2383-9902(eISSN)
한국미생물학회 (The Microbiological Society of Korea)
권호 표지

Trichoderma koningii에서 분리한 $\beta$-glucosidase의 작용양상

The Action Mode of $\beta$-glucosidase Purified from Trichoderma koningii

  • 발행 : 2000.03.01
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초록

Trichoderma koningii ATCC 26113에서 분비되는 효소인 $\beta$-glucosidase를 cellobiose, sophorose, laminaribiose 및 gentiobiose 등의 기질과 반응시킨 후 효소의 transglycosylation 반응 산물을 분석하였다. 각각의 기질로부터 생성된 이당체(dimer)들을 HPLC로 분리하고 $^(1)H$-NMR spectroscopy를 통하여 분석하였다. Cellobiose를 기질로 사용하여 효소와 반응시켰을 때 그 산물에는 laminaribiose, sophorose 및 gentiobiose가 포함되었음을 확인할 수 있었다. Laminaribiose, sophorose 및 gentiobiose를 기질로 사용하였을 경우에 효소는 transglycosylation 반응을 통하여 새로운 $\beta$-glycosidic 결합을 갖는 이당체들을 생성하였다. 효소반응에 의하여 누적되는 이당체의 양은 생성속도보다는 분해속도에 의하여 결정되는 것으로 나타났다.

We have examined the mode of transglycosylation, catalyzed by an extracellular $\beta$-glucosidase purified from Trichoderma koningii ATCC 26113, using cellobiose, sophorose, laminaribiose and gentiobiose as substrates. The dimers separated from the reaction mixture by HPLC were analyzed by $^(1)H$-NMR spectroscopy. When cellobiose was subjected to the action of the $\beta$-glucosidase, the products included laminaribiose, sophorose and gentiobiose. When laminaribiose, sophorose or gentiobiose was used as a substrate, the $\beta$-glucosidase accumulated transglycosylation products possessing different types of $\beta$-glycosidic linkages from the original one. The amount of dimers accumulated as reaction proceeded seemed to be dependent on the velocity of hydrolysis but not on that of formation.

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