YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

Characteristics of Tetanus Toxoid Loaded in Biodegradable Microparticles

파상풍 톡소이드를 함유한 생체분해성 미립구의 특성

  • 김지윤 (식품의약품안전청 생물학평가부 세균제제과) ;
  • 김수남 (식품의약품안전청 생물학평가부 세균제제과) ;
  • 백선영 (식품의약품안전청 생물학평가부 세균제제과) ;
  • 이명숙 (식품의약품안전청 생물학평가부 세균제제) ;
  • 민홍기 (식품의약품안전청 생물학평가부 세균제제과) ;
  • 홍성화 (식품의약품안전청 생물학평가부 세균제제과)
  • Published : 2000.08.01
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Abstract

Biodegradable microspheres made from poly-lactide-co-glycolide polymers have been considered as a new delivery system for single-dose vaccine. Purified tetanus toxoid (TT) was encapsulated in poly-lactide(PLA) and poly-lactide-co-glycolide (PLGA) microparticles using a solvent evaporation method in a multiple emulsion system (water-in oil-in water). The morphology of 77-loaded microparticles was spherical and the suface of them was smooth. The particle size was in a range of 2-10. Protein loading efficiency was 68-97.8%. PLGA (85:15) microparticle showed the highest efficiency. Protein release pattern was influenced by polymer molecular weight and composition. The release rate of PLA(Mw 100,000) microsphere was higher than any other microspheres. In consequence of the hydrolysis of PLGA(50:50) microspheres, environmental pH decreased from 7.4 to 5.0. The PLA, PLGA (75:25) and PLGA (85:15) microshperes showed no significant pH change. The antigenicity or n in microshperes was assayed by indirect sandwich ELISA using equine polyclonal tetanus antitoxin for capture antibody and human polyclonal tetanus antitoxin for primary antibody. The antigenicity of TT in PLA (Mw 100,000), PLGA(50:50, Mw 100,000) and PLGA (75:25, Mw 73,300) after 30 days incubation showed 54, 40.9 and 76.7%, respectively.

Keywords

References

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