한국초지조사료학회지 (Journal of The Korean Society of Grassland and Forage Science)
- 제20권2호
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- Pages.103-108
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- 2000
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- 2287-5824(pISSN)
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- 2287-5832(eISSN)
Development of Transgenic Orchardgrass (Dactylis glomerata L.) Using Agrobacterium tumefaciens
- Lee, Hyoshin (College of Agriculture, Kyungpook National University) ;
- Lee, Byung-Hyun (College of Agriculture, Kyungpook National University) ;
- Jinki Jo (College of Agriculture, Kyungpook National University)
- 발행 : 2000.06.01
초록
To produce of transgenic orchardgrass, the seed-derived calli of orchardgrass (Dactylis glomerata L.) co-cultivated with Agrobacterium turnefaciens EHAlOl harboring binary vector pIG121-Hm were selected with hygromycin and then transferred onto N6 regeneration medium containing 1 rngl l of NAA, 5 rngl l of kinetin, 250 rngl l of carbenicillin and 50 mg/ l of hygromycin. The efficiency of transformation was differed on cultivars, that is, 'Potomac' appeared 12% of transformation efficiency while 'Amba' did 5.5%. The addition of acetosyringone during co-cultivation was a key to successhl transformation of orchardgrass. Transgene fragments were identified by PCR analysis and the constitutive expression of GUS gene was confirmed by Northern blot analysis. (Key words : Acetosyringone, Agrobacterium tumefaciens, Orchardgrass (Dactylis glomerata L.), Transformation)
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