The Korean Journal of Soil Zoology (한국토양동물학회지)

The Korean Society of Soil Zoology (한국토양동물학회)
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Distribution and Some Properties of the Alkaline Phosphatase from the Midgut of the Earthworm, Eisenia andrei

지렁이 중장에서 분리한 Alkaline Phophatase의 분포와 특성에 관한 연구

  • 박희우 (중앙대학교 생명과학과) ;
  • 조성진 (중앙대학교 생명과학과) ;
  • 조표연 (중앙대학교 생명과학과) ;
  • 이명식 (중앙대학교 생명과학과) ;
  • 이규석 (관동대학교 의과대학 해부학교실) ;
  • 박순철 (중앙대학교 생명과학과)
  • Published : 2000.06.01
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Abstract

The distribution and some properties of alkaline phosphatase (ALP) were investigated in the midgut of the earthworm, Eisenia andrei. The ALP activity appeared to be highly polarized toward the luminal side of epithelium, with minor ALP activity in chloragogue tissue. The epithelial and chloragogeneous tissues contained approximately 85 and 15% of total intestinal ALP activity, respectively. The optimal temperature was approximately 37$^{circ}C$ and isoelectricpoint was estimated to be 4. The treatment of neuraminidase and PtdIns-PLC failed to change the migration rate of ALPs. Also, these ALPs appeared to have a wide range of substrate specificity. The relationship between the properties and physiological significances of the midgut ALPs in Eisenia andrei was discussed.

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