Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Beef Tallow Hydrolysis by Immobilized Lipase

  • Kim, Dong-Joon (Department of Biological Engineering, Inha University) ;
  • Shin, Dong-Hoon (Graduate School of Biotechnology, Korea University) ;
  • Hur, Byung-Ki (Department of Biological Engineering, Inha University) ;
  • Kim, Eun-Ki (Department of Biological Engineering, Inha University)
  • Published : 2000.12.01
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Abstract

Beef tallow, which is an industrial lipid substrate, was hydrolyzed by lipase immobilized on a high-density polyethylene (HDPE) powder. Ethanol pre-washing process affected the immobilization efficiency. Half-life of storage of the HDPE at $4^{\circ}C$ was 150 days. And after 10 times of repeated use, more than 50% of initial activity remained. An apparent Michaelis constant ($K_m$) and maximum velocity ($V_{max}$) were 2.7M, and 1.4 mmol/min/l for immobilized lipase, and 0.5 M, and 1.9 mmol/min/l for soluble lipase, respectively.

Keywords

References

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