Ultrastructural Aspects of the Mixed Infections of Watermelon Mosaic Potyvirus Isolated from Pumpkin and Cucumber Green Mottle Mosaic Tobamovirus from Watermelon

  • Cho, Jeom-Deog (School of Agricultural Biotechnology, Seoul National University) ;
  • Kim, Jeong-Soo (Vegetable Breeding Division, National Horticultural Research Institute) ;
  • Park, Hong-Soo (Plant Pathology Division, National Institute of Agricultural Sciences, Technology) ;
  • La, Yong-Jun (School of Agricultural Biotechnology, Seoul National University) ;
  • Kim, Kyung-Soo (Department of Plant Pathology, University of Arkansas, Fayetteville)
  • 발행 : 2000.08.01

초록

Mixed infection of watermelon mosaic potyvirus II isolated from pumpkin (WMV-P) and cucumber green mottle mosaic tobamovirus from watermelon (CGMMV-W) caused extremely severe symptoms such as progressive silting and death of watermelon plants. Single infections of either WMV-P or CGMMV-W on the same hosts produced only vein clearing and/or mosaic on the upper leaves. In cells infected with WMV-P, potyvirus-characteristic inclusions of pinwheels, scrolls and cylindrical inclusions were present in the cytosol. Parallel arrays of virus particles in the tonoplast were also common. In cells infected with CGMMV-W, virus particles occurred as stacked-bands of scattered randomly in the cytosol and vacuoles in all type cells. Many cells also contained vesiculated mitochondria with fibril-containing vesicles. Cells infected mixedly with WMV-P and CGMMV-W contained structural features that were not observed in cells infected singly with the two viruses. A particle of WMV-P potyvirus was surrounded by evenly spaced nine particles of CGMMV-W tobamovirus, which made a unique nonagon ring. The angled layers having $60^{\circ}$-$135^{\circ}$ were alternating layer, herringbone, crosshatching and ladder figures.

키워드