KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Separation and Recovery of Cyclodextrin Glucanotransferase Using Aqueous Two-Phase Systems

수성2상계를 이용한 Cyclodextrin Glucanotransferase 분리 및 회수

  • 김진현 ((주)삼양제넥스 생명공학연구소) ;
  • 홍승서 ((주)삼양제넥스 생명공학연구소) ;
  • 이현수 ((주)삼양제넥스 생명공학연구소)
  • Published : 2000.12.01
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Abstract

Cyclodextrin Glucanotransferase(EC 2.4.1.19 : 1,4-${\alpha}$-glucano) transferase, cyclizing; CGTase) can be separated and recovered in an aqueous two-phase system composed of poly(ethylene glycol)(PEG)/dextran and PEG/salt. In an aqueous two-phase system consisting of PEG 35000 (5%) and dextran T2000 (7%), all cell and debris were collected at the interphase. CGTase partitioned to the denser dextran phase at an yield of 83.4%. On the other hand, in an aqueous two-phase system consisting of PEG 35000 (10%) and sodium phosphate (15%), CGTase partitioned to the denser salt phase at an yield of 95.5%. In order to recover CGTase using an aqueous two-phase system, the PEG/salt system proved to be more efficient than the PEG/dextran system in terms of yield and cost.

Keywords

References

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