Evaluation of Allergenicity for Fish and Method for Reduction of Allergenicity by Food Technological Treatment

생선의 Allergen성 판정과 Allergen성을 감소시키는 가공학적 방법

In this research the results showed that Evans blue stain causes vascular permeation at antibody injection site by the passive cutaneous anaphylaxis(PCA) screening of octpus minor so we concluded. Octopus minor causes allergy. Psedosciaena Polyactis, Raja Kenojei, Metapenaeus joyneri also showed allergenicity. Microwave and autoclaving appeared to reduced allergenicity(up to 99%) during the technological treatment processing. On the other hand, UV light didn't seem to change the protein structure of allergens affect the allergenicity. Therefore, the technological treatment processing of fish such as canning and microwave would possibly reduce the allergenicity. Among the ultrafiltration fraction of Octopus minor, Pseudosciaena Polyactis, Raja Kenojei and Metapenaeus joyneri, those fraction over 100,000 contained allergen and those under 100,000 and when screening allergenic fish went through 10,000~100,000 ultrafiltration, only the fraction of over 100,000 showed the anaphylactis activity for PCA. However whether screening fish would cause anaphylaxis in human or not is questionable. The future clinical experiment will verify this result with clinical experiment patients.

In this research the results showed that Evans blue stain causes vascular permeation at antibody injection site by the passive cutaneous anaphylaxis(PCA) screening of octpus minor so we concluded. Octopus minor causes allergy. Psedosciaena Polyactis, Raja Kenojei, Metapenaeus joyneri also showed allergenicity. Microwave and autoclaving appeared to reduced allergenicity(up to 99%) during the technological treatment processing. On the other hand, UV light didn't seem to change the protein structure of allergens affect the allergenicity. Therefore, the technological treatment processing of fish such as canning and microwave would possibly reduce the allergenicity. Among the ultrafiltration fraction of Octopus minor, Pseudosciaena Polyactis, Raja Kenojei and Metapenaeus joyneri, those fraction over 100,000 contained allergen and those under 100,000 and when screening allergenic fish went through 10,000∼100,000 ultrafiltration, only the fraction of over 100,000 showed the anaphylactis activity for PCA. However whether screening fish would cause anaphylaxis in human or not is questionable. The future clinical experiment will verify this result with clinical experiment patients. 본 연구 결과로 낙지의 PCA검색으로써 항체주사 부위에 Evan's Blue 착색으로 인하여 vascular permation이 일어나 allergynicity 반응이 인정되므로 낙지는 allergy가 있다고 할 수 있다. 조기, 홍어, 새우도 역시 allergenicity성이 나타난 것을 볼 수 있었다. 또한 공정 중 microwave와 autoclaving은 4가지 수산식품 낙지, 조기, 홍어, 새우의 allergenicity를 현저히 감소시키는 것으로 나타났다. 반면에 자외선은 단백질로 구성된 allergen의 구조는 크게 변화시키지 못하는 것으로 보여진 것을 알 수 있다. 그러므로 생선의 가공방법은 통조림 가공이나 microwave 처리가 allergenicity성을 감소시킬 수 있는 것으로 보여진다. 낙지, 조기, 홍어, 새우의 한외여과 fraction중 고분자인 100,000이상에서만 allergenicity가 나타났다. 따라서 이 allergen들의 분자량은 100,000 이상으로 추정되며 검색된 allergynicity 생선을 10,000∼100,000으로 한외여과 하였을 때 100,000 이상의 fraction에서만 PCA에 의해 allergenicity가 있는 것으로 인정되었다. 그러나 이 검색된 생선이 anaphylaxis가 인간에게서까지도 반응할지는 의문이다. 이 연구의 결과는 차후 임상실험환자에 의하여 최종 검증되어야 한다.