Effectof of Hormonal Treatment of Induced Maturation and Ovulation in the Spotted Halibut, Verapser variegatus

호르몬 처리에 의한 범가자미, Verasper variegatus 의 성숙과 배란유도

  • Baek, Hea-Ja (Aquaculture Division, National Fisheries Research and Development Institute) ;
  • Kim, Yoon (Aquaculture Division, National Fisheries Research and Development Institute) ;
  • An, Cheul-Min (Aquaculture Division, National Fisheries Research and Development Institute) ;
  • Cho, Kee-Chae (Aquaculture Division, National Fisheries Research and Development Institute) ;
  • Myeong, Jeong-In (Aquaculture Division, National Fisheries Research and Development Institute) ;
  • Lee, Nam-Ju (Aquaculture Technology Laboratory Section, Sung-Gi Industry)
  • 백혜자 (국립수산진흥원 양식개발과) ;
  • 김윤 (국립수산진흥원 양식개발과) ;
  • 안철민 (국립수산진흥원 양식개발과) ;
  • 조기채 (국립수산진흥원 양식개발과) ;
  • 명정인 (국립수산진흥원 양식개발과) ;
  • 이남주 (성지실업(주))
  • Published : 2000.03.01

Abstract

Hormone induction in maturation and ovulation was carried out with spotted halibut (Verasper variegatus) which normally does not spawn spontaneously in captivity. Prior to spawning females were treated with human chorionic gonadotropin(HCG 265-678 IU/kg BM) 17 ${\alpha}$-hydroxy 20-${\beta}$-dihydroprogesterone (17 ${\alpha}$ 20${\beta}$ OHP 0.5-1.0${\mu}$g/kg BW) and luteinizing hormone-releasing hormone analogue pellet (LHRHa pellet 63-81 ${\mu}$g/kg BW) In all the trials HCG injection succeeded in inducing ovulation at 265-300 IU/kg BW. HCG at 620 or 678 IU/kg BW was ineffective in inducing a good rate of ovulation. Final maturation and ovulation were not stimulated by treatment of 17 ${\alpha}$ 20${\beta}$OHP and LHRHa pellet. These results suggest that HCG injection (around 300 IU/kg BW) is more effective compared with LHRHa pellet implantation.

사육수조내 자연산란이 이루어지지 않는 범가자미 암컷을 대상으로 호르몬 처리에 의안 인위적 성숙.배란 유도 실험이 이루어졌다. 산란시기에 범가자미 체중 kg당 HCG 265~678 IU, 17${\alpha}$ 20${\beta}$ OHP 0.5~1.0${\mu}$g 농도의 복강주사와 LHRHa 63~81 ${\mu}$g의 펠렛을 투여한 결과, HCG농도 약 300 IU가 범가자미의 최정성숙과 배란유도에 효과적인 것으로 나타났다. 17${\alpga}$ 20${\beta}$ OHP와 대조군에서는 난의 성숙이 거의 진행되지 않았으며, LHRHa 펠렛 실험군에서도 난의 최종성숙과 배란이 일어나지 않았다. 따라서 범가자미 암컷의 경우 현재 많이 사용되고 있는 LHRHa 펠렛 투여보다도 저농도의 HCG (dir 300 IU/kg 체중)o 주사가 난의 최종성숙과 배란에 더 효과적인 것으로 나타났다.

Keywords

References

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