Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Cytotoxicity of Daucus Carota L. on Various Cancer Cells

인체 암세포에 대한 당근 추출 성분의 세포독성효과

  • 배송자 (신라대학교 식품영양학과) ;
  • 한은주 (신라대학교 식품영양학과) ;
  • 노승배 (양산대학 식품가공과)
  • Published : 2000.02.01
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Abstract

We investigated the cytotoxic effects of roots and seeds Daucus carota L. on HepG2, HeLa, MCF7, SW626, C6 and NB41A3 cell lines by the MTT assay. Among extracts, the ethylacetate partition layer (DCMEA) of root of Daucus carota L. showed the strongest cytotoxic effects on HepG2, HeLa, C6 and NB41A3 cell lines. On the other hand, methanol(DCM), n-ethylacetate(DCMEA) and n-butanol(DCMB) extract of the seeds of Daucus carota L. also showed significant cytotoxic activities for all six cell lines. $\beta$-carotene, a well-known main component of Daucus carota L. was also tested for its cytotoxic effect. However, in all six cell lines, $\beta$-carotene falied to show significant cytotoxicity. Therefore, the anticancer effect of DCMEA of root fo Daucus carota L. and DCM, DCMH, DCMEA and DCMB extracts of seeds may be caused by components other than $\beta$-carotene. Futher studies are under way to isolate the compounds responsible for the significant cytotoxic activity.

Keywords

References

  1. Cancer Res. v.51 Cancer prevention : Recent progress and futrue opportunities Weinstein, I.B.
  2. Cancer facts and figues American Cancer Society
  3. Elements of human cancer Cooper, G.M.
  4. J. Natl. Cancer Inst. v.66 The causes of cancer-Quantitative estimate of avoidable risks of cancer in the united states today Doll, R.;Peto, R.
  5. J. Kor. Soc. Food Sci. Nutr. v.27 Antimutagenicity and cytotoxicity of Artemisia iwayomogi Kitamura extracts Ham, S.S.;Chung, C.K.;Lee, J.H.;Choi, K.P.;Jung, S.W.;Kim, E.J.
  6. Yakhak Hoeli v.40 Chemical components of Acanthopanax divaricatus and anticancer effect in leaves Yook, C.S.;Rho, Y.S.;Seo, S.H.;Leem, J.Y.;Han, D.R.
  7. J. Kor. Soc. Food Sci. Nutr. v.25 The antimutagenic anc anticancer effect of Taxus cuspidata extract Hwang, B.H.;Zhao, J.L.;Choi, K.P.;Jung, S.W.;Kim, E.J.;Ham, S.S.
  8. J. Nutr. Growth Cancer v.4 Beta-carotene and smoking Gerster, H.
  9. Int.. J. Cancer v.44 Serum concentration of beta-carotene and intake frequency of green-yellow vegetables aming healthy inhabitants in Japan Shinbata, A.;Sasaki, r.;Ito, Y.;Reuler, J.B.;Broudy, V.C.
  10. N. Engl. J. Med. v.330 The α-tocopherol, β-carotene cancer prevention study group Owens, W.C.;Cross, K.W.
  11. Proc. Nat. Acad. Sci. v.1 Potential role of the antioxidant vitamins and carotenoids in governing human aging rate Culter, R.G.
  12. Cancer Res. v.48 Feasibility of drug screening with panels of human tumor cell lines using a microoculture tetrazolium assay Michael, C.A.;Dominic, A.S.;Anue, M.
  13. Cancer Res. v.47 Evalution of a tetrazolium based semiautomated colorimetric assay : assessment of chemosensitivity testing Carmichael, J.;De Graff, W.G.;Gazder, A.F.;Minna, J.D.;Mitchell, J.B.
  14. J. Kor. Cancer Assoc. v.25 Effects of verapamil, tamoxifen and cyclosporin A for the modulation of multidrug resistance in human lung cancer cell lines Kim, J.H.;Kim, B.S.;Choi, J.J.;Kim, K.M.;Yoo, N.C.;Choi, J.H.;Lim, H.Y.;Roh, J.K.;Lee, K.S.;Kim, B.S.
  15. Cancer Res. v.52 Differential effects of dietary linoleic acid on mouse skin-tumor promotion and mammary carcinogenesis Fischer, S.M.;Leyton, L.J.;Lee, M.L.;Locnislcar, M.;Belury, M.A.;Maldve, R.E.