KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Extraction and Purification of Bioactive Materials from Agaricus blazei Fruiting Bodies

아가리쿠스 버섯에서 생리활성물질의 추출 및 정제

  • Published : 2000.06.01
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Abstract

${\beta}$-Glucan a kind of polysaccharide which is particularly abundant in Agaricus blazei is known as the bioactive materials especially anticancer agents. The process development of the isolation and the purification process of water soluble ${\beta}$-glucans from A. blazei was achieved. and the process operation variables were optimized. Crude polysaccharides (CR.PS) were obtained from A. blazei by hot water extraction filtration solvent precipitation dialysis and freeze drying. Neutral and acidic fraction of polysaccharides were separated from crude polysaccharides by ion chromatography and then high molecular weight and low molecular weight fraction were separated from neutral fraction by gel chromatography. Quantitative and qualitative analysis of each compounds were performed with FT-IR NMR spectroscopy. Based on these analysis the optimal conditions of temperatures operating time organic solvent volume for precipitation and dialysis time were determined.

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References

  1. Edible Mushroom and Their Cultivation Chang, S. T;P.G. Miles
  2. Kor. J. Mycol v.10 no.3 Antitumor Components of Cryptoporus volvatus Kim, B. K., J. E. Robbers, K. S. Chung, H. S. Chung;E. C. Choi
  3. Nature New Biol v.238 Anticom Plementary Activity of Antitumor Polysaccharides Sugihara, T., Y. Yoshioka;K. Nishioka
  4. Agric. Biol. Chem v.54 no.11 Antitumor Activity and Some Properties of Water Soluble Polysaccharides from the Fruiting Body of Agricus blazei Murill Mizuno, T
  5. Jpn. J. pharmacol v.66 Inhibitory Action of a (1→6)-β-D-glucan-Protein Complex Isolated from Agaricus blazei Murill on Meth a Fibrosarcoma-Bearing Mice and its Antiumor Mechanism Itoh, H., H. Amano;H. Noda
  6. Agric. Biol. Chem v.54 no.11 Antitumor Acivity and Some Properties of Water-insoluble Hetero-glycans from "Himematsutake"the Fruiting Body of Agaricus blazei Murill Mizuno, T., R. Inagaki, T. Kanao;T. Hagiwara
  7. Carbohydrate Research v.186 Fraction and Antitumor Activity of the Water Insoluble Residue of Agarcus blazei Fruiting Bodies Kawagishi, H., R. Inagaki;T. Kanao
  8. Nippon Nogeikagaku Kaishi v.58 no.9 Fractionatiion, Structural Features and Antitumor Activity of Water-soluble Polysaccharide from 'Reishi', the Fruit Body of Ganoderma lucidum Mizuno, T., M. Kato;M. Shimizu
  9. Analyt. Biochem v.72 A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-dye Binding Bradford, M. M
  10. Carbohydraate Research v.100 High-field, 13C-N.M.R. Spectroscopy of D-Glucans, Amylopectin, and Glycogen Dais, P;A. S. Perlin
  11. Carbohydrate Research v.258 NMR Spectral Analysis of a Water-insoluble (1→3)-D-Glucan Isolated from Saccharomyces cerevisiae Harry E. E;T. Brain