Development of Ceramic Media for Yeast Immobilization

효모 고정화용 세라믹 담체의 개발

  • Published : 2000.06.01

Abstract

Support media for yeast immobilization was prepared from a porous volcanic rock used as a moisturizer in orchid growing. The rock was broken to the size of 2-3 mm and burned at $600^{\circ}C$ in a furnace in order to remove organic materials blocking the pores or treated with HCI solution or NaOH solution to remove the inorganic dirts by dissolving. Even through both the acid and the akali solution were effective the latter was not recommendable because it broke the pore structure by dissolving the elements of the media. This media was mainly consisted of SiO2 with $Al_2O_3$ as a minor component and CaO and K2O as trace elements. It had the finely developed pores of $15-80\mu\textrm{m}$size. Yeast immobilization capacity of this media was about $5{\times108}$ cells/ml bed which is large enough to be used for the practical applications. Yeast immobilization capacities of Alumina and Cordierite were much smaller than that of silica-based media. Scanning electron micrograph of Cordierite and Alumina showed uneven surfaces and small size of pores in contrast to relatively smooth surface and large pores of silica based media which means that smooth surface and large pores are desirable for the good adsorption of microbes on the media.

난초를 키우는 화분에 보습용으로 사용되는 다공성 화산석을 2-3 mm크기로 파쇄한 후 $600^{\circ}C$에서 1시간 태워서 가연성 물질 을 제거하고 IN의 염산용액으로$60-70^{\circ}C$에서 2시간 이물질을 녹여댄 후 세척할 경우 효모 고정화에 적당한 담체를 얻을 수 있었다 이 때 염산 용액 대신 NaOH 용액을 세척용액으로 사용 할 수 있으나 담체 구성성분의 용출이 있을 수 있으므로 용액 농도, 처리온도 및 시간에 주의하여야 한다 이렇게 제조된 담체 는 기공의 크기가 $15-80\mu\textrm{m}$이고 충전 밀도는 0.4-0.6 g/mL 였으며 $SiO_2$가 주성분이었고, $Al_2O_3$가 소량함유되어 있었으며, CaO, MgO가 미량 함유되어 있었다. 이 담제의 구조와 효모 고 정화 능력을 국내외 기업에서 제조한 실리카 및 알루미나 재질 의 담체의 경우와 비교한 결과 고상포말법으로 제조한 실리가 담체의 구조 및 고정화능력($5{\times108}$ cells/mL bed)과 비슷한 결 과를 보여 효모 고정화용 담체로 손색이 없었다.. Cordierite 담체 와 알루미나 담체는 고정화능이 실리카 담체의 50% 이하로 낮 았는데 이는 기공의 표면이 매끄럽지 못했기 때문으로 효모가 쉽게 흡착하기 위해서는 가능한 한 매끄러운 표면이 바람직하다 는 사실을 알 수 있었다.

Keywords

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