KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
권호 표지

The Effects on Escherichia coli and Plasmid DNA Using Ultrasoft X-ray

Ultrasoft X-ray의 Escherichia coli균과 plasmid DNA에 대한 영향

  • Published : 2000.02.01
Download PDF
⟨ Previous Next ⟩

Abstract

We studied the effect of ultrasoft X-ray obtained from the Pohang Light Source (PLS), on the mutation of E. coli and the damage of plasmid. After irradiation, the supercoiled plasmid DNA converted to the relaxed-form, and then to the linear-form. We transformed the irradiated plasmid DNA and isolated $\beta$-galactosidase mutants. We also isolated $\beta$-galactosidase mutants from the directly irradiated cells. There were preferred mutational sites on DNA induced by ultrasoft X-ray.

포항 선형 가속기로부터 얻은 ultrasoft X-선이 대장균의 돌연변이 유도와 plasmid DNA 변이에 주는 영향을 알아보았다. 빔을 조사함에 따라 supercoil 형태의 plasmid DNA가 relaxed 형태로 변하고, 그후 선형으로 변해 감을 확인하였다. 빔을 조사한 plasmid DNA를 E. coli에 형질전환하여 $\beta$-galactosidase의 돌연변이 균을 분리하였고, 빔을 직접 조한 E. coli균으로부터도 $\beta$-galactosidase의 돌연변이 균을 얻었다. 변이된 plasmid DNA와 변이 대장균들의 plasmid DNA의 염기 부분을 변화를 확인하였으며 이 빔에 의해 주로 변이가 일어나는 부분이 있음을 알았다.

Keywords

References

  1. Induction and rejoining of DNA double-strand breaks in chinese hamster V79-4 cells irradiated with characteristic aluminum k and copper I ultrasoft x rays. Radiat. Res. v.148 Botchway S.W.;D.L. Stevens;M.A. Hill;T.J. Jenner;P. O'neill
  2. Proc. Nail. Acad. Sci. USA v.69 Nonchromosomal antibrotic resitnce in bacteria: genetic transformation of Escherichia coli by R-factor DNA Cohen S.N.;A.C.Y. Chang;L. Hsu
  3. Int. J. Radiat. Biol. v.64 Measurement of DNA damage by electrons with energies between 25and 4000 eV Folkard M.;K.M. Prise;B. Vojnovic;S. Davis;M.J. Roper;B.D. Michael
  4. Int. J. Radiat. Biol. v.50 Effectiveness of 1.5 KeV aluminium K and 0.3 KeV carbon K characteristic X-rays at inducing DNA Double-strand breaks in yeast cells Frankenberg D.;D.T. Goodhead;M. Frankenberg-Schwager;R. Harbich;D.A. Banse;R.E. Wilkinson
  5. DNA repair and muragenesis Friedberg E.C.;G.C. Walker;W. Siede
  6. Int. J. Radiat. Biol. v.66 DNA damage induced by vacuum and soft X-ray photons from synchrotron radiation Hieda K.
  7. Int. J. Radiat. Biol. v.70 Single- and double-strand breaks in pBR322 plasmid DNA by monochromatic X-rays on and off the K-absorption peak of phosphorus Hieda K.;T. Hirono;A. Azami;M. Suzuki;Y. Furusawa;H. Maezawa;N. Usami;A. YokoYa;K. Kobayashi
  8. Comparative analysis of deletion and base-change mutabilities of Escherichia coli B sttains differing in DNA repair capacity (wild-type, uvrA-, polA-, recA-) by various mutagens. Mutat. Res. v.27 Ishii Y.;S. Kondo
  9. Appl. Radiat. Isot. v.45 Damage induced by monochromatic synchrotron x-rays at the resonance absorption energy of intracellular phosphorous in yeast cells Jin T.;D.E. Watt;K. Kobayashi
  10. Enzyme Microbiol. Technol. v.17 Dextransucrase constitutive mutants of Leuconostoc mesenteroides B-1299 Kim D.;J.F. Robyt
  11. Biotechnol. Techniq. v.11 Development of constitutive dextransucrase hyper-producing mutants of Leuconostoc mesenteroides using the synchrotron radiation in the70~1000 cV region Kim D.;D.W. Kim;J.H. Lee;K.H. Park;L.M. Day;D.F. Day
  12. Int. J. Radiat. Biol. v.59 Effects of k-shell x-rays absorption of intracellular phosphorus on yeast cells Kobayashi K.;K. Hieda;H. Maezawa;Y. Furusawa;M. Suzuki;T. Ito
  13. Chem. Res. Toxical. v.10 Differential effects of DNA supercoiling on radical-mediated DNA strand breaks Lamarr W.A.;K.M. Sandman;J.N. Reeve;P.C. Dedon
  14. Principles of biochemistry Lehninger A.;D.L. Nelson;M.M. Cox
  15. Int. J. Radiat. Biol. v.59 Strand breaks in plasmid DNA, natural and brominated, by low-energy X-rays Menke H.;W. Kohnlein;S. Joksch;A. Halpern
  16. Int. J. Radiat. Biol. v.66 Action spectra for single- and double- strand break induction in plasmid DNA : studies using synchrotion radiation Michael B.D.;K.M. Prise;M. Folkard;B. Vojnovie;B. Brocklehurst;I.H. Munro;A. Hopkirk
  17. Nucleic Acids Res. v.4 DNA double-strand breaks and alkali-labile bonds produced by bleomycin Povirk L.F.;W. Wubker;W. Kohnlein;F. Hutchinson
  18. Radiat. Res. v.117 Measurement of DNA damage and cell killing in chinese hamster V79 cells irradiated with aluminum characteristic ultrasoft X rays Prise K.M.;M. Folkard;S. Davies;B.D. Michael
  19. J. Phoochem. photobiol. B: Biol. v.28 Effects of vacuum UV and UVC radiation on dry E. coli plasmid pUC19-Ⅰ. Inactivation, lacZ muttation induction and strand breaks Wehner I.;G. Horneck