Crystal vilet 색소분해능이 소실된 Citrobacter sp. 의 분리 및 특성

Isolation and Characterization of Citrobacter sp. Mutants Defective in Decolorization of Crystal Violet

  • Kim, Ji-Yoon (Division of Biotechnology, Faculty of Natural Resources and Life Science, Dong-A University) ;
  • Kim, Kyung-Woon (Division of Biotechnology, Faculty of Natural Resources and Life Science, Dong-A University) ;
  • Park, Yong-Lark (Division of Biotechnology, Faculty of Natural Resources and Life Science, Dong-A University) ;
  • Cho, Young-Su (Division of Biotechnology, Faculty of Natural Resources and Life Science, Dong-A University) ;
  • Lee, Young-Choon (Division of Biotechnology, Faculty of Natural Resources and Life Science, Dong-A University)
  • 발행 : 2000.08.01

초록

To identify genes involved in the decolorization of crystal violet, we isolated random mutants generated by transponson insertion in crystal violet-declorizing bacterium, Citrobacter sp. The resulting mutant bank yielded mutants with six distinct phenotypes, and Southern hybridization with a Tn5 fragment as a probe showed a single hybridized with six distinct phenotypes, and Southern hybridization with a Tn5 fragment as a probe showed a single hybridized band in the mutants Ctg 2, 5 an 6, whereas two and three bands were detected in Ctg1, 4 and 3, respectively. Tn5-inserted genes were isolated and the DNA sequence flanking Tn5 was determined. From comparison with a sequence database, putative protein product encoded by ctg 5 was identified as E. coli maltose transproter(Mal G) homolog, whereas the deduced amino acid sequence of the other ctg genes did not show any significant similarity with any DNA or protein sequency. Therefore, these results indicate that the other ctg genes except ctg 5 encode new proteins responsible for decolorization of crystal violet.

키워드

참고문헌

  1. Proc. Natl. Acad. Sci. USA v.83 A GTP-binding protein of Escherichia codi has homology to yeast RAS protein. Ahnn, J.;P. E. March;H. E. Takiff;M. Inouye
  2. Enzyme Microb. Technol. Microbial decolorization of triphenylmethane and azo dyes by Citrobacter sp. An, S.-Y.;K.-W. Kim;Y.-L. Choi;Y.-S. Cho;S.-K. Min;I.-H. Cha;C.-H. Kim;Y.-C. Lee
  3. Mutat. Res. v.58 Cytogenetic toxicity of gentian violet and crystal violet on mammalian cells in vitro. Au, W.;S. Pathak;C. J. Collie;T. C. Hsu
  4. Mutat. Res. v.66 Further study of the genetic toxicology of gentian violet Au, W.;M. A. Butler;S. F. Bloom;T. S. Matmey
  5. Enzyme Microb. Technol. v.22 Biodegradation of triphenylmethane dyes. Azmi, W.;R. K. Sani;U. C. Banerjee
  6. Biochem. J. v.17 The inhibition of 1,1,1-trichloro-2,2-bis-(p-chlorophenyl)ethane (DDT) dehydrochlorinase and glutathione S-aryltransferase in grass-grub and horusefly preparations. Balabaskaran, S.;J. N. Smith
  7. Biores. Technol. v.58 Microbial decolorization of textile-dye-containing effluents : a review Banat, I. M.;P. Nigam;D. Singh;R. Marchant
  8. Mol. Biol. Rev. v.62 Maltose/maltodextrin system of Escherichia coli : transport, metabolism, and regulation. Boos, W.;H. Shuman
  9. Nature. v.349 The GTPase superfamily: conserved stucture and molecular mechanism. Bourne, H. R.;D. A. Sanders;F. McCormick
  10. Appl. Environ. Microbiol. v.54 Biodegradation of crystal violet by the white rot fungus Phanerochaete chrysosporium. Bumpus, J.A.;B. J. Brock
  11. J. Biol. Chem. v.265 Expression and characterization of RNase III and Era proteins. Chen, S.-M.;H. E. Takiff;A. M. Barver;G. C. Dubois;J. C. A. Bardwell;D. L. Court
  12. Control of messenger RNA stability. RNA processing and degradation by RNase III. Court, D.;J. Belasco(ed.);G. Brawerman(ed.)
  13. Mol. Microbiol. v.7 Membrane topology of MalG, an inner membrane protein from the maltose transport system of Escherichia coli. Dassa, E.;S. Muir
  14. J. Biol. Chem. v.266 Purification and charaterization of the membrane-associated components of the maltose transport system form Escherichia coli. Davidson, A. L.;H. Nikaido
  15. Biochem. Pharmacol. v.45 Inhibition of glutathione S-transferases from rat liver by basic triphenymethane dyes. Dabnam, P. ;S. Glanville;A. Clark
  16. Encyclopedie of Chemical Technolgy v.8 Dyes and dyes intermediates Gregory, P.;J. L. Kroschwitz(ed.)
  17. J. Mol. Biol. v.233 Genetic analysis of periplasmic binding protein dependent transport in E. coli: each lobe of the maltose-binding protein interacts with a different subunit of MalFGK2 membrane transport complex. Hor, L. I.;H. A. Shuman
  18. J. Pharm. Sci. v.89 Effect of molecular structure on the performance of photochemical purging of autologous bone marrow grafts from residual tumor cells. Indig,G. L.;G. S. Anderson;M. G. Nichols;J. A. Barlett;W. S. Mellon;F. Sieber
  19. Drugs of choice. Drugs for intestinal parasitism. Kean, B. H.;D. W. Haskins;W. Modell(ed.)
  20. Poult. Sci. v.55 A study of the feasibility of the use of gentian violet as a fungistat for poultry feed. Kingsland, G.V.;J. Anderson
  21. J. Bacteriol. v.17 GTPase-dependent signalling in bacteria: characterization of a membrane-binding site for era in Escherichia coli. Lin, Y. P.;J. D. Sharer;P. E. March
  22. J. Mol.Biol. v.3 A procedure for the isolation of deoxyribonucleic acid from microorganisms. Marmur, J
  23. J. Biol. Chem. v.268 The ATP-binding cassette (ABC) transporter for maltose/maltodextrin of Salmonella typhimurium. Characterization of ATPase activity associated with the purified MalK subunit. Morbach, S.;S. Tebbe;E. Schneider
  24. EMBO J. v.16 Subunit interaction in ABC transporter, A conserved sequence in hydrophobic membrane proteins of periplasmic permeases define sites of interaction with the helicel domain of ABC subunits. Mourez, M.:M. Hofnung;E. Dassa
  25. Environ. Sci. Technol. v.14 Aromatic amines in and near the Buffalo River. Nelson, C.R.;R. A. Hites
  26. Molecular cloning: a laboratory manual. Sambrook, J.;E. F. Fritsch;T. Maniatis
  27. Gene v.56 A series of Tn5 variants with various drug-resistance markers and suicide vector for transposon mutagenesis. Sasakawa, C.;M. Yoshikawa
  28. Biotechnol. Lett. v.20 Decolorization of artificial dyes by peroxidase from the white-rot fungus Pleurotus ostreatus. Shin, K.-S.;C.-J. Kim
  29. Mol. Microbiol. v.12 Characterization of the autophosphorylation of Era, an essential GTPase in E. coli. Sood, P.;C. G. Lerner;T. Shimamoto;Q. Lu;M. Inouye
  30. J. Bacteriol. v.171 Genetic analysis of the rnc operon of Escherichia coli. Takiff, H.;S.-M. Chen;D. L. Court
  31. Curr. Microbiol. v.30 Decolorization of triphenymethane dyes by the birds nest fungus Cyathus bulleri. Vasdev, K.;R. C. Kuhad;R. K. Saxena
  32. Escherichia coli and Salmonella typhimurium: cellular and molecular biology. General recombination in Escherichia coli. Weinstock, G.;F. C. neidhardt(ed.);J. L. Ingraham(ed.);K.B. Low(ed.);B. Magasanik(ed.);M. Schaechter(ed.);H. E. Umbarger(ed.)