Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Development of DNA Chip Microarrayer

  • Yoon, Sung-Ho (Department of Chemical Engineering and BioProcess Engineering Research Center, Korea Advanced Institute of Science and Technology) ;
  • Choi, Jong-Gil (Department of Chemical Engineering and BioProcess Engineering Research Center, Korea Advanced Institute of Science and Technology) ;
  • Lee, Sang-Yup (Department of Chemical Engineering and BioProcess Engineering Research Center, Korea Advanced Institute of Science and Technology)
  • Published : 2000.02.01
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Abstract

A microarrayer system was developed mainly for manufacturing DNA chips. The 3-axis robot was designed to automatically collect samples from 96-or 384-well microtiter plates using up to 16 simultaneously moving pens and to deposit them on a surface-modified slide glass. This is followed by a wash/dry operation in a clean station. The cycle is repeated with a new set of samples, This system can deposit cDNA or oligonucleotides with spot intervals of $150{\;}\mu\textrm{m}$ and the spot size of $80\mu\textrm{m}$, thus allowing a high density DNA chip containing about 5,000 spots per $\textrm{cm}^2$. The entire procedure is controlled by the Visual C++ program that was written in our laboratory by using a personal computer with Pentium 100 CPU.

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