Abstract
Eighty-eight strain3 o~methicillin resistant Stopllylococcus awecis were Isolated from pus (64.7%); spuhm (26.2%), blood, fluid, andurine of 83 patients at Dong-A Hospital in P~~san to invesligate theil-coagulase typ- Ing, and multi-drug resistaut ppattems. The presence of niec A gene confe~~ing melhicillin resistance was tested by polymerase chain reaction (PCR) with uwo mec A gene specific primers using purified clromosonlal DNA as templates. DNA fragments of expected size wel-e detected frorn 86 strains, but not from two strains. !i coagulase typmg, the 86 isolates were assigned to 5 coagulase lypes, I, 11, lll. 1V, VI, VII, VIlI, but there was no isolate helong lo type V. The most abundant coagulase type was type TI(50 %), lollowed by type IV Rest ofthe coagulase types were ininor; ranging fmm 4.5 to 12.5 '% Most of the type I1 ~netlucillin resistant Stapl\ulcorneryiococcus nwem (MRSA) strams were isolated from the generd sulzely ward, but major strains of type IV were Isolated from the otorhinolq~ngology of the hospital's outpatient clinic center. All of the 88 st~nins were sensitive to vancomycin and teicoplanin, but 71 (81%) strains showed multi-drug resitant to penicillin, cephalotl~n, eiythroinycin, gentan~ycin, imipenem, clindamycin, ciprofloxacin and ooxacillin. Yo relationship was found between the antibiotic resistance pattems aud the coagulase typing patterns.
부산시 동아대학병원으로 내원한 83명의 환자 가건물로부터 methcillin 내성 Stphylococcus aureus(MRSA) 88 균주를 분리하고 응고 효소의 유형별 분석과 다약제 내성의 상호 관계를 연구하였다. 농(64.7%), 객담(26.2%), 기타 혈액, 장액 및 뇨로부터 균주가 분리되었고 mec A 유전자에 특이적인 primer 5'-AAAATCGATGGTAAAGGTT-GGC-3'와 5'-AGTTCTGCAGTACCGGATTTGC3'를 사용하여 PCR을 한결과 86균주에서 mec 유전자가 확인되었다. 응고효소형은 제 III형이 50%m IV형이 12.5%, III형이 6.8%, I, VII, VIII형이 4.5%로 제 II형이 가장 많았으며 제 V형은 전혀 분리되지 않았다. 응고효소형의 병동별 분포는 일반외과에서 제 II형이, 이비인후과 외래에서는 제 IV형이 우점종으로 분리되었다. 항균제 감수성 시험결과, vancomycin과 teicoplanin에서 전균주가 감수성을 나타내었었고 penionillin, cephalothin, erythromycin, gentamycin, imipenem, clindamycin, ciprofloxacin과 oxacillin등 8가지의 항균제에 대하여 동시내성을 나타낸 균주가 71주 (81%)였다. 항균제 내성과 응고효소형과의 상관관계는 없었다.
