Journal of Microbiology

The Microbiological Society of Korea (한국미생물학회)
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Use of the Yeast 1.5-Hybrid System to Detect DNA-Protein-Protein Interaction

  • Kim, Sook-Kyung (Laboratory of Fermantation Microbiology and Biotechnology, School of Biololgical Sciences, Seoul National University, Seoul 151-742) ;
  • Han, Jin-Hee (Laboratory of Fermantation Microbiology and Biotechnology, School of Biololgical Sciences, Seoul National University, Seoul 151-742)
  • Published : 2000.03.01
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Abstract

Escherichia coli F plasmid partition apparatus is composed of two trans-acting proteins (SopA and SopB) and one cis-acting DNA sequence (sopC). The SopB-sopC complex has been suggested to serve a centromere-like function through its interaction with chromosomally encoded proteins which remain to be identified. In this paper, we are introducing a new yeast 1.5-hybrid system which assembles the two-hybrid and one-hybrid system as a mean to find and additional component of the F plasmid partition system, interacting with DNA (sopC)-bound SopB protein. The results indicates that this system is a promising one, capable of selecting an interacting component.

Keywords

References

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