Journal of Microbiology

The Microbiological Society of Korea (한국미생물학회)
권호 표지

A Recombinant Mous GM-CSF Protein Expressed as an Inclusion Form Shows Colony Stimulating Activity

  • Hyun Joo Youn (Department of Microbiology, College of Natural Sciences, Inje University, Kimhae 621-749, Korea) ;
  • Jin-Kyoo Kim (Department of Microbiology, College of Natural Sciences, Changwon National University, Changwon 241-773, Korea) ;
  • Eun-Jung Sohn (Department of Microbiology, College of Natural Sciences, Changwon National University, Changwon 241-773, Korea) ;
  • Soo-O Lee (Department of Microbiology, College of Natural Sciences, Changwon National University, Changwon 241-773, Korea) ;
  • Choon-Taek Lee (Department of Internal Medicine, College of Medicine, Seoul National University, Seoul 110-744, Korea)
  • Published : 2000.03.01
Download PDF
⟨ Previous Next ⟩

Abstract

Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a pleiotropic hematopoietic growth factor and an activator of lmature myeloid cells and recombinant GM-CSF is increasingly under clinical studies for the treatment of various diseases including cancer, infectious diseases and hematopoietic diseases. We constructed a reconbinant mouse GM-CSF expression plasmid with pelB leader sequence and His. Tag under T7 promoter control, and showed that the construct produced a 20 kDa recombinant protein in 8M urea. We also showed that the 20 kDa recombinant protein prepared in 8M urea sitmulated colony formation in vitro, indicating that the recombinant mGM-CSF can be renatured to its native form to show the colony stimulating activity.

Keywords

References

  1. Cancer v.88 Short term treatment with Escheria coli recombinant human granulocyte-macrophage-colony stimulating factor prior to chemotherapy for Hodgkin disease Aglietta,M.;F.Montemurro;F.Fagioli;C.Volta;B.Botto;M.Cantonetti;V.Racanelli;L.Teofili;R.Ferrara;S.Amadori;G.L.Castoldi;F.Dammacco;A.Levis
  2. Am.J.Nephrol. v.20 Granulocyte-Macrophage Colony Stimulatinf Factor as an adjuvant to Hepatitis B vaccination in maintenance hemodialysis patients Anandh,U.;B.Bastani;S.Ballal
  3. Ann.Rev.of Biochem. v.59 Cytokines: coordinators of immune and inflammatory response Arai,K.;F.Lee;A.Miyajima;S.Miyatake;N.Arai;T.Yokota
  4. Clin. Exp. Immunol. v.119 Exacerbation of acute inflammatory arthritis by the colony-simulating factors CSF-1 and granulocyte macrophage (GM)-CSF:evidence of macrophage infiltration and local proliferation Bischof, R.J.;D. Zafiropoulos;J.A. Hamilton;I.K. Campbell
  5. Eur.J.Cancer 35 v.35 no.SUP.3 GM-CSF as adjuvant for immunotherapy with bispecific antibodies Elsasser,D.;H.Stadick;J.G.van de Winkel;T.Valerius
  6. Lymphokines and Interferons: A Practical Approach. Assays for interleukin3 and other myeloid colony-stimulating factors Graland,J.;Clemens(ed.);Morris AG(ed.);Gearing AJH(ed.)
  7. Eur.J.Cancer v.35 no.SUP.3 GM-CSF can cause t cell activation: results of seuential chemo-immunotherapy Groenewegen,G.;G.C.de Gast
  8. Proc. Natl. Acad. Sci. USA v.91 Specific human granulocyte-macrophage colony stimulating factor antagonists Hercus, T.R.;C.J. Bagley;B. Cambareri;M. Dottore;J.M. Woodcock;M.A. Vadas;M.F. Shannon;A.F. Lopez
  9. Br.J.Dermatol. v.142 Successful treatment of aphthous ulcerations in AIDS patients using topical granulocyte-macrophage colony-stimulating factor Herranz,P.;J.R.Arribas;a.Navarro;J.M.Pena;J.Gonzalez;F.A.Rubio;M.Casado
  10. Adv. Intern. Med. v.45 Cytokine therapy of mycobacterial infections Holland, S.M.
  11. Proc.Natl.Acad.Sci.USA. v.95 CTLA-4 blockade synergizes with tumor-derived granulocyte-macrophage colony-stimulating factor for treatment of an experimental mammary carcinoma Hurwitz,A.A.;T.F.Y.Yu;D.R.Leach;J.P.Allison
  12. Clin.Immunol. v.93 Treatment with tumor necrosis factor-alpha and granulocyte-macrophage colony-stimulating factor increases epidermal Langerhans' cell numbers in cancer patients Janik,J.E.;L.L.Miller;W.C.Kopp;D.D.Taub;H.Dawson;D.Stevens;P.Kostboth;B.D.Curti;K.C.Conlon;B.K.Dunn;S.E.Donegan;R.Ullrich;W.G.Alvord;B.L.Gause;D.L.Longo
  13. Exp. Hematol. v.27 Granulocyte macrophage colony-stimulating factor and interleukin-3 regulate chemokine and chemokine receptor expression in bone marrow macrophages Jarmin, D.I.;R.J. Nibbs;T. Jamieson;J.S. de Bono;G.J. Graham
  14. Eur.J.Cancer v.35 no.SUP.3 Use of granulocyte-macrophage colony stimulating factor (GM-CSF) in prevention and treatment of jungal infections Jones,T.C.
  15. J.Viral Hepat. v.6 Granulocyte-macrophage colony stimulating factor enhances the efficacy of hepatitis B Virus vaccine in previously unvaccinated haemodialysis patients Kapoor,D.;S.R.Aggarwal;N.P.Singh;V.Thakur;S.K.Sarin
  16. J.Inf.Dis. v.181 Granulocyte-macrophage colony stimulating factor augments phagocytosis of Mycobacterium avium complex by hyman immunodeficiency virus type 1-infected monocytes/macrophages in vitro and in vivo Kedzierska,K.;J.Mak;a.Mijch;I.Cooke;M.Rainbird;S.Roberts;G.Paukovics;D.Jolley;A.Lopez;S.M.Crowe
  17. Proc.Natl.Acad.Sci.USA. v.88 Stimulation of granulopoiesis by transforming growth factor β: Synergy with granulocyte/macrophage colony stimulating factor Keller,J.R.;S.E.W.Jacobsen;K.T.Sill;L.R.Ellingsworth;F.W.Ruscetti
  18. Scand. J. Immunol. v.40 Catabolism of the murine IgG1 molecule: evidence that both CH2-CH3 domain interfaces are required for persistence of IgG1 in the circulation of mice Kim, J.K.;M.F. Tsen;E.S. Ward
  19. Eur.J.Cancer v.35 no.WUP.3 Febrile neutropenia: prophylactic and therapeutic use of GM-CSF Mangi,M.H.;A.C.Newland
  20. Eur.J.Cancer v.35 no.SUP.3 Local lowdose of soluble GM-CSF significantly augments an immune response against tumour antigens in man Mellstedt,H.;J.Fagerberg;A.Osterborg
  21. Cell. Mol. Ther. v.5 Intradermal injection of granulocyte-macrophage colony-stimulating factor (GM-CSF) in patients with metastatic melanoma recruits dendritic cells Nasi, M.L.;P. Lieberman;K.J. Busam;V. Prieto;K.S. Panageas;J.J. Lewis;A.N. Houghton;P.B. Chapman
  22. Proc.Natl.Acad.Sci.USA. v.90 Granulocyte-macrophage and macrophage colony stimulating factors differentially regulates integrin expression on cultured human macrophages Nichilo,M.O.D.;G.F.Burns
  23. J.Invest.Derm. v.113 Granulocyte-macrophage colony stimulating factor gene transfer to dendritic cells or epidermal cells augments their antigenpresenting function including induction of anti-tumor immunity Ozawa,H.;W.Ding;H.Torii;J.Hosoi;K.Seiffert;K.Campton;N.R.Hackett;N.Topf;R.G.Crystal;R.D.Granstein
  24. Biosci.Biotechnol.Biochem. v.63 Induction of GM-CSF production of macrophages by advanced glycation end products of the Maillard reaction Sasaki,T.;S.Horiuchi;M.Yamazaki;S.Yui
  25. Adv.Perit.Dial. v.14 Granulocyte macrophage-colony stimulating factor stimulates secretion of chemoattractive cytokines by peritoneal macrophages of CAPD patients Schafer,K.;S.Stoteknuel;P.Schollmeyer;G.J.Dobos
  26. Proc. Natl. Acad. Sci. USA v.95 Vaccination with irradiated autologous melanoma cells engineered to secrete human granulocyte-macrophage colony-stimulating factor generates potent antitumor immunity in patient with metastatic melanoma Soiffer, R.;T. Lynch;M. Mihm;K. Jung;C. Rhuda;J.C. Schmollinger;F.S. Hodi;L. Liebster;P. Lam;S. Mentzer;S. Singer;K.K. Tanabe;A.B. Cosimi;R. Duda;A. Sober;A. Bhan;J. Daley;D. Neuberg;G. Parry;J. Rokovich;L. Richards;J. Drayer;A. Berns;S. Clift;L.K. Cohen;R.C. Mulligan;G. Dranoff
  27. Proc.Natl.Acad.Sci.USA. v.92 A regulatory element in the promoter of the human granulocyte-macrophage colony stimulating factor gene that has related sequences in other T-cell expressed cytokine genes Staynov,D.Z.;D.J.Cousins;T.H.Lee
  28. J.Surg.Oncol. v.72 Treatment of liver metastases from colon carcinoma with autologous tumor vaccine expressing granulocyte-macrophage colony-stimulating factor Suh,K.W.;S.Piantadosi;H.A.Yazdi;D.M.Pardoll;H.Brem;MA Choti
  29. Eur.J.Cancer v.35 no.SUP.3 GM-CSF and stimulation of monocyte/macrophage function in vivo relevance and in vitro observations Williams,M.A.;S.M.Kelsey;A.C.Newland