Radiation Oncology Journal
- Volume 18 Issue 2
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- Pages.138-149
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- 2000
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- 2234-1900(pISSN)
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- 2234-3156(eISSN)
The Frequency of Chromosomal Aberrations of Peripheral Lymphocytes according to Radiation Dose and Dose Rate
선량 및 선량률 변화에 따른 말초혈액 임파구의 염색체 이상의 빈도
- Jeong Tae Sik (Department of Radiation Oncology Kosin Medical College and Medical Center) ;
- Baek Heum Man (Department of Radiation Oncology Kosin Medical College and Medical Center) ;
- Shin Byung Chul (Department of Radiation Oncology Kosin Medical College and Medical Center) ;
- Moon Chang Woo (Department of Radiation Oncology Kosin Medical College and Medical Center) ;
- Kim Mi Hyang (Departments of Clinical Pathology Kosin Medical College and Medical Center) ;
- Lee Yong Hwan (Departments of Preventive Medicine Kosin Medical College and Medical Center) ;
- Yum Ha Yong (Department of Radiation Oncology Kosin Medical College and Medical Center)
- 정태식 (고신대학교 의과대학 복음병원 방사선종양학과교실) ;
- 백흠만 (고신대학교 의과대학 복음병원 방사선종양학과교실) ;
- 신병철 (고신대학교 의과대학 복음병원 방사선종양학과교실) ;
- 문창우 (고신대학교 의과대학 복음병원 방사선종양학과교실) ;
- 김미향 (고신대학교 의과대학 복음병원 임상병리학과교실) ;
- 이용환 (고신대학교 의과대학 복음병원 예방의학과학교) ;
- 염하용 (고신대학교 의과대학 복음병원 방사선종양학과교실)
- Published : 2000.06.01
Abstract
Purpose : It was studied that the relationship between radiation dose, dose rate and the frequency of chromosomal aberrations in peripheral lymphocytes. Methods and Materials : Peripheral lymphocytes were irradiated in vitro with 6 MeV X-ray at dose ranges from 50 cGy to 800 cGy. The variations of the frequency of chromosomal aberrations were observed according to different radiation dose rate from 20 cGy/min to 400 cGy/min at constant total dose of 400 cGy which it was considered as factor to correct biological radiation dose measurement. Results : The yields of lymphocytes with chromosomal aberrations (dicentric chromosome, ring chromosome, acentric fragment pairs) are 0% at 50 cGy, 9% at 100 cGy, 20% at 200 cGy, 27% at 300 cGy, 55% at 400 cGy, 88% at 600 cGy, and 100% at 800 cGy. The value of Ydr is 0.000 at 50 cGy, 0.093 at 100 cGy, 0.200 at 200 cGy, 0.354 at 300 cGy, 0.612 at 400 cGy, 2.040 at 600 cGy, and 2.846 at 800 cGy. The relationship between radiation (D) and the frequency of dicentrlc chromosomes and ring Chromosomes (Ydr) can be expressed as Ydr=0.188
목적 : X-선 조사 후 이동원 염색체와 환형 염색체의 빈도가 피폭 선량과 비례하는지를 확인하고, X-선 조사시 선량률의 변화가 생물학적 선량 측정에 영향을 주는지를 찾아서 보정인자로 작용하는지를 알아보았다. 대상 및 방법 : 28세의 건강한 성인남자에서 2회에 걸쳐 각각 16 ml와 18 ml의 전혈을 정맥으로부터 무균채취하여 이미 해파린(heprin) 처리되어 있는 10 ml의 vacutainer (삼화공사, 한국)에 2 ml씩 분주하였다. 분주한 혈액 시료를 1개는 정상대조 군으로 16개는 실험 군으로 총 17개를 실험에 사용하였으며, 방사선 조사는 조사야 10
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