The Journal of Internal Korean Medicine (대한한방내과학회지)

The Society of Internal Korean Medicine (대한한방내과학회)
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Effects of Samul-tang on Nitric Oxide Induced-cytotoxicity in C6 Glial Cell

Nitric Oxide에 의해 유발된 C6 glial 세포독성(細胞毒性)에 대한 사물탕(四物湯)의 방어효과(防禦效果)

  • Kim, Do-Hwan (Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Kim, Seung-Mo (Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Cho, Han-Gook (Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Cha, Yong-Seok (Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Heo, Yun (Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Cho, Kwang-Ho (Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Moon, Byung-Soon (Professional Graduate School of Oriental Medicine, Wonkwang University)
  • 김도환 (원광대학교 한의학전문대학원) ;
  • 김승모 (원광대학교 한의학전문대학원) ;
  • 조한국 (원광대학교 한의학전문대학원) ;
  • 차용석 (원광대학교 한의학전문대학원) ;
  • 허윤 (원광대학교 한의학전문대학원) ;
  • 조광호 (원광대학교 한의학전문대학원) ;
  • 문병순 (원광대학교 한의학전문대학원)
  • Published : 2000.12.30
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Abstract

The water extracts of Samul-tang(SMT) has been used for treatment of ischemic brain damage in Oriental traditional medicine. However, little is known about the mechanism by which the water extracts of SMT rescues brain cells from ischemic damages. To elucidate the protective mechanism on ischemic induced cytotoxicity, I investigate the regulation of LPS and PMA induced iNOS expression in C6 glial cells. LPS and PMA treatment for 72 h in C6 glial cells markedly induce nitric oxide(NO), but treatment of the cells with the water extracts of SMT decrease. dose dependently nitrite formation. In addition, LPS and PMA treatment for 72 h induce severe cell death and LDH release in C6 glial cells. However treatment of the cells with the water extracts of SMT dose not induce significant changes compare to control cells. Furthermore, the protective effects of the water extracts of SMT is mimicked by treatment of $N^{G}MMA$, a specific inhibitor of NOS. LPS and PMA induced iNOS activation in C6 glial cells cause chromosomal condensation and fragmentation of nuclei by caspase activation. The treatment of the cells with the water extracts of SMT may suppress apoptosis via caspase inhibition by regulation of iNOS expression. Taken together, I suggest that the protective effects of the water extracts of SMT against ischemic brain damages may be mediated by regulation of iNOS during ischemic condition.

Keywords

References

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