The Journal of Internal Korean Medicine (대한한방내과학회지)

The Society of Internal Korean Medicine (대한한방내과학회)
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The Protective Effects of Sunghyangjeongki-San on Middle Cerebral Artery Occlusion

성향정기산(星香正氣散)이 뇌허혈(腦虛血)을 유발(誘發)시킨 백서(白鼠)의 신경전달물질(神經傳達物質)에 미치는 영향(影響)

  • Yea, Gyeong-Uk (Department of Circulatory Internal Medicine, College of Oriental Medicine, Kyungsan University) ;
  • Park, Chi-Sang (Department of Circulatory Internal Medicine, College of Oriental Medicine, Kyungsan University) ;
  • Lee, Eun-Ju (Department of Circulatory Internal Medicine, College of Oriental Medicine, Kyungsan University) ;
  • Song, Jee-Hea (Department of Pharmacology, College of Oriental Medicine, Kyungsan University) ;
  • Kim, Mi-Ryeo (Department of Pharmacology, College of Oriental Medicine, Kyungsan University) ;
  • Cho, Jung-Sook (Department of Pharmacology, College of Medicine, Dongguk University) ;
  • Kim, Young-Ho (Department of Pharmacognosy, College of Pharmacy, Chungnam National University) ;
  • Park, Chang-Gook (Department of Pharmacology, College of Oriental Medicine, Kyungsan University) ;
  • Yang, Chae-Ha (Department of Pharmacology, College of Oriental Medicine, Kyungsan University)
  • 예경욱 (경산대학교 한의과 대학 심계내과학 교실) ;
  • 박치상 (경산대학교 한의과 대학 심계내과학 교실) ;
  • 이은주 (경산대학교 한의과 대학 심계내과학 교실) ;
  • 송지혜 (경산대학교 한의과 대학 약리학 교실) ;
  • 김미려 (경산대학교 한의과 대학 약리학 교실) ;
  • 조정숙 (동국대학교 의과대학 약리학 교실) ;
  • 김영호 (충남대학교 약학대학 생약학 교실) ;
  • 박창국 (경산대학교 한의과 대학 심계내과학 교실) ;
  • 양재하 (경산대학교 한의과 대학 약리학 교실)
  • Published : 2000.08.30
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Abstract

Objectives : The aim of this study is to investigate that Sunghyangjeongki-San which has been frequently medicated in the early stage of stroke can protect against ischemic damage in rat brain Methods : Extracellular levels of amino acids(glutamate, aspartate, GABA, glycine, taurine, tyrosine, alanine), organic acids(pyruvate, lactate), and cerebral infarction volume were measured at the striatum of rats subjected to permanant focal cerebral ischemia induced by 2 hours of middle cerebral artery occiusion(MCAO). Rats were orally administered with Sunghyangjeongki-San at 30mins before MCAO and the microdialysate was collected by intracerebral microdialysis three times before MCAO and six times after MCAO at 20mins interval and analyzed by HPLC. After a microdialysis study, the brain was sliced and stained with cresyl violet buffer for the measurement of cerebral infarcted area and volume by image analyzer system Results : The concentrations of glutamate, aspartate, and tyrosine known as excitatory neurotransmitters were significantly decreased in Sunghyangjeongki-San group compared with control group, The concentrations of GABA, glycine, taurine and alanine known as inhibitory neurotransmitters were significantly increased in Sunghyangjeongki-San group compared with control group. The concentrations of pyruvate and lactate showed little significant change in Sunghyangjeongki-San group compared with control group. The measurement of cerebral infarcted area and volume by image analyzer system were significantly decreased in Sunghyangjeongki-San group compared with control group. Conclusions : Sunghyangjeongki-San can affect on protecting against cerebral ischemia by regulating extracellular levels of excitatory and inhibitory amino acid neurotransmitters and improve the conditions of the patients in the early stage of stroke.

Keywords

References

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