THE JOURNAL OF KOREAN ORIENTAL ONCOLOGY (대한한방종양학회지)

Korean Association of Traditional Oncology (대한암한의학회)
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Effects of the Gamihyangsayukgunjatang on Radioprotection and Apoptosis in Small Intestines of Mice

가미향사육군자탕(加味香砂六君子湯)이 생쥐 소장(小腸)에서 방사선조사(放射線照射) 후(後) 보호효과(保護效果)와 Apoptosis에 미치는 영향(影響)

  • Lee, Tae-Eob ;
  • Kim, Jin-Sung (Dept. of Oriental Medicine, Graduate School, Kyung Hee University) ;
  • Yoon, Sang-Heup (Dept. of Oriental Medicine, Graduate School, Kyung Hee University) ;
  • Ryu, Bong-Ha (Dept. of Oriental Medicine, Graduate School, Kyung Hee University) ;
  • Park, Dong-Won (Dept. of Oriental Medicine, Graduate School, Kyung Hee University) ;
  • Ryu, Ki-Won (Dept. of Oriental Medicine, Graduate School, Kyung Hee University)
  • Published : 2000.12.30
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Abstract

The present study was performed in order to evaluate the effects of the gamihyangsayukgunjatang on radioprotection and apoptosis in small intestines of mice after whole body irradiation. Two hundred forty mice were divided into 40 groups according to the radiation dose and the gamihyangsayukgunjatang treatment. The extracts of the herbal medicines were orally administered to each group differently before and/or after irradiation. The gamihyangsayukgunjatang treated groups were divided into 3 groups. Sample Ⅰ was the group treated with the gamihyangsayukgunjatang for 3 days before the radiation, sample Ⅱ was the group treated with the gamihyangsayukgunjatang for 3 days after the radiation. Sample Ⅲ was the group treated with the gamihyangsayukgunjatang for both 3 days before and after the radiation. To analyze the crypt survival, the micradony survival assay was used according to the Withers and Elind's method. To analyze the apptosis, the TUNEL assay was done. The results obtained are a follows : 1. From the microcolony survival assay, the gamihyangsayukgunjatang treated groups showed the radioprotective effect with a statistical significance(p<0.05), as compared to the control group. Comparing the radioprotective effect among the 3 groups, sample III was statistically more significant than sample I and II (p<0.05). Sample I showed no effect. In accordance with the research mentioned above, it is suggested that the radioprotective effect of the gamihyangsayukgunjatang is more useful for the treatment of the radiation injury rather that the prevention. 2. The results of the TUNEL assay showed that the apoptotic index in the gamihyangsayukgunjatang treated group was slightly decreased with no effectiveness, as compared to the control group. According to the above results, it could be suggested that the gamihyangsayukgujatang has a prominent protective effect in mice intestines against the radiation damage. However, the radioprotective effect does not seem to be related to inhibition of the apoptosis.

Keywords

References

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