A Method for Simultaneous Analysis of Phthalate Esters and Di-(2-ethylhexyl) Adipate Migrated from PVC Wraps into Fatty Food

PVC Wraps에서 지방함유 식품으로 이행되는 Phthalate esters 및 Di-(2-ethylhexyl) adipate의 동시 분석법

A method for simultaneous quantitative determination of plasticizers such as diethyl phthalate(DEP), di-n-propyl phthalate(DprP), di-n-butyl phthalate(DBP), di-n-pentyl phthalate(DPP), butylbenzyl phthalate(BBP), di-(2-ethylhexyl) phthalate(DEHP), dicyclohexyl phthalate(DCHP) and di-(2-ethylhexyl) adipate(DEHA), which are suspected as endocrine disruptors, in food was studied. A analysis method was optimized for the quantification of plasticizers in Jjambbong, which is a kind of fatty noodle, by using GC/FID. The detection limits of DEP, DprP, DBP, DPP, BBP, DEHP, DCHP and DEHA were 3.5, 5.3, 2.2, 2.2, 7.2, 1.7, 1.9 and 3.0 mg/kg, respectively. Much higher recovery was obtained by extraction with acetone/n-hexane(1:1) rather than hexane solvent system. The recovery of DEP, DprP, DBP, DPP, BBP, DEHP, DCHP and DEHA were 72.7, 85.9, 91.4, 97.1, 100.8, 103.2, 104.3 and 95.8% after 4 time extractions, respectively. The migration of plasticizers from PVC wraps into PVC wrap covered Jjambbong was conducted after shaking(120 rpm) for 30min at room temperature with chosen solvent system. It was found that the migration level of DEHA were 577, 706, 770 mg/kg into Jjambbong, respectively, while the other plasticizers were not observed.

식품에서 내분비계장애추정물질로 의심되는 DEP, DprP, DBP, DPP, BBP, DEHP 및 DCHP 등과 같은 phthalate esters와 DEHA를 동시 정량 분석하였다. GC/FID를 사용하여 지방 함유 식품의 일종인 짬뽕에서 가소제를 정량하기 위한 최적의 분석방법을 검토하였다. Phthalate esters인 DEP, DprP, DBP, DPP, BBP, DEHP, DCHP와 DEHA의 검출한계는 각각 3.5, 5.3, 2.2, 2.2, 7.2, 1.7, 1.9 와 3.0 mg/kg이었다. Acetone/n-hexane(1:1) 추출용매 system을 사용하여 4회 추출하고 HP-1701 column이 장착된 GC/FID를 사용했을때의 phthalate esters인 DEP, DprP, DBP, DPP, BBP, DEHP, DCHP와 DEHA의 회수율이 각각 72.7, 85.9, 91.4, 97.1, 100.8, 103.2, 104.3 및 95.8% 이었다. 3종의 업소용 PVC wrap으로 짬뽕을 각각 덮은채 30분간 shaking(120회/분)한 후 이행되는 가소제 함량을 조사하였다. 3종의 PVC wrap에서 짬뽕으로 DEHA가 각각 577, 706, 770 mg/kg 이행되어 검출되었으며 다른 phthalate esters는 검출되지 않았다.