Korean Journal of Food Science and Technology (한국식품과학회지)
- Volume 32 Issue 6
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- Pages.1221-1226
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- 2000
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- 0367-6293(pISSN)
Property Comparison of Polyclonal Anti-Soy Protein Antibodies Produced for ELISA
효소면역측정법을 위한 다클론 항대두단백 항체의 생산 및 특성비교
- Shon, Dong-Hwa (Korea Food Research Institute) ;
- Kim, Hyun-Jung (Korea Food Research Institute) ;
- Yun, Sung-Seob (Maeil Dairy Industry Co., Ltd.)
- Published : 2000.12.30
Abstract
Specific antibodies were produced to develope the enzyme-linked immunosorbent assay for analysis of soy proteins and the properties of the antibodies were compared. Isolate soy protein(ISP), and ISP heated with SDS and urea (ISP(SU)), acidic subunits(AS) of 11S globulin were immunized to produce polyclonal antibodies. By using competitive indirect ELISA(ciELISA), the reactivities of the antibodies toward soy proteins treated with different methods were investigated and shown as
대두단백질의 분석을 위한 효소면역측정법을 개발하고자 특이항체를 생산하고 그 항체의 특성을 비교하였다. 분리대두단백(ISP), ISP를 SDS와 urea의 첨가하에 열처리한 것(ISP(SU)), 11S 글로불린의 acidic subunit(AS)를 각기 면역하여 다클론 항체를 생산하였다. 간접 경합 효소면역측정법(ciELISA)을 실시하여 처리를 달리한 대두단백질에 대한 이들 항체의 반응성을 조사하였여