Screening of Deoxynivalenol Producing Fungi from Greenhouse Horticulture by Enzyme Amplification System Immunoassay

Enzyme Amplification System Immunoassay에 의한 시설원예산물의 Deoxynivalenol 생성곰팡이의 검색

In order to evaluate the safety of greenhouse horticulture products in Korea, we carried out this work by screening of Fusarium species, which produce deoxynivalenol (DON) from greenhouse horticulture in Western Gyeongnam and Northern Gyeongbuk, Korea. For this study, high sensitive enzyme-linked immunosorbent assay, ALP/NADP method, was applied to detection of DON by enzyme amplification system. From 192 samples of greenhouse horticulture soil and its products, 103 isolates of Fusarium species were obtained. The isolates were cultured at 28C for 15 days and the cultured mediums were extracted by ethyl acetate. The production of DON was verified by thin layer chromatography (TLC). As the results of TLC, 8 strains were identified as DON producing strain. We screened potential producers of DON by ALP/NADP. The levels of DON production were shown from 0.007 to 1.21 g/ml of YES medium. The maximum DON producing strain No. 32-D-3 was isolated from soil in Namhae, Korea. In conclusion, the above results indicate that DON producing fungi contaminated greenhouse horticulture products in Korea. Therefore, further studies are required to accumulate more detailed data about the contamination of DON in various cereals.

시설원예산물 중 DON 생성능이 있는 균주의 오염 여부를 조사하기 위해 진주를 비롯한 서부 경남 일원과 경북 안동 근교에서 총 192점의 시료를 분리하여 균 분리를 실시한 결과 Fusarium속 103균주를 분리하였다. 분리된 균을 YES배지로 $28^{\circ}C$, 15일 배양한 후 ethyl acetate로 추출하여 thin layer chromatography(TLC)법으로 확인한 결과 DON 생성 균주 8개가 확인되었다. Enzyme amplification system immunoassay법을 이용하여 DON 생성 균주 8개를 정량한 결과 검출범위는 $0.007-1.21\;{\mu}g/mL$ 이었다. 이 중 남해 토양에서 분리한 32-D-3 균주가 $1.21\;{\mu}g/mL$로 가장 높았다.