Journal of The Korean Society of Grassland and Forage Science (한국초지조사료학회지)
- Volume 19 Issue 4
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- Pages.303-308
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- 1999
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- 2287-5824(pISSN)
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- 2287-5832(eISSN)
Callus Induction from Seeds of Birdsfoot trefoil and Plant Regeneration on BOi2Y Medium
버즈풋 트레포일 종자로부터 캘러스 유도 및 BOi2Y 배지에서 식물체 재분화
- Kim, Ki-Yong (National Livestock Research Institute) ;
- Rim, Yong Woo (National Livestock Research Institute) ;
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Choi, Kee Jun
(National Livestock Research Institute) ;
- Sung, Byung Ryul (National Livestock Research Institute)
- Published : 1999.12.30
Abstract
The conditions for callus formation and plant regeneration were confirmed in birdsfoot trefoil (Lotus corniculatus L.). Among SH (Schenk and Hildebrandt), MS (Murashige and Skoog) and N6 medium (Chu), SH medium was highest degree of efficiencies respectively in callus formation and plant regeneration. In this study, we determined volume of hormones and other compounds appended in media. For callus formation, only
버즈풋 트레포일 (Lotus corniculatus L. cv. Empire) 종자로부터 직접 캘러스를 유도하고, 형성된 캘러스로부터 식물체를 재분화하는 조건을 확립하였다. 캘러스 유도시 사용한 SH (Schenk and Hildebrandt), MS (Murashing and Skoog), N6 (Chu) 배지중에서 SH 배지가 캘러스 유도에 유리한 것으로 나타났으며, 캘러스 유도 및 중식시에는 2,4-D