The Journal of Korean Academy of Prosthodontics (대한치과보철학회지)
- Volume 37 Issue 6
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- Pages.717-729
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- 1999
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- 0301-2875(pISSN)
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- 2005-3789(eISSN)
CYTOTOXICITY OF DENIAL CAST BASE METAL ALLOYS ON HUMAN ORAL KERATINOCYTES
구강점막 상피세포에 대한 치과 주조용 비귀금속 합금의 세포독성
- Choi, Young-Jin (Department of Prosthodontics, College of Dentistry, Yonsei University) ;
- Yook, Jong-In (Department of Oral Pathology, College of Dentistry, Yonsei University) ;
- Chung, Moon-Kyu (Department of Prosthodontics, College of Dentistry, Yonsei University)
- Published : 1999.12.01
Abstract
Although many studies on the cytotoxicity of the dental cast base metal alloys and their components have been carried out, the results are rather conflicting because of the different type of cells used and the various experimental procedures taken. Recently a number of scientists have claimed that it would be preferable to focus on the use of cells from relevant specific location of the human bodies. Consequently, the primary cultured oral keratinocyte derived from oral mucous along with nickel chloride and several of widely used dental cast base metal alloys(two Ni-Cr alloys and one Co-Cr alloy)in domestic were selected for this study, from which 1) The amounts of released metal ions were determined using atomic absorption spectrometry, 2) The cytotoxicity of nickel chloride and dental cast base metal alloys was evaluated via MTT assay, and finally, 3) The amounts of released metal ions and the cytotoxicity of nickel chloride were correlated with the cytotoxicity of dental cast base metal alloys And, the results were summarized as follows; 1. Nickel ion from Ni-Cr alloys and Cobalt ion from Co-Cr alloys resulted in maximum releasing rate during first 2h hours, followed by a decrease in releasing rate with time. Chromium ion were found to be minimal in all alloys. 2. In cytotoxic test. with