Korean Journal of Veterinary Research (대한수의학회지)
- Volume 39 Issue 3
- /
- Pages.513-522
- /
- 1999
- /
- 2466-1384(pISSN)
- /
- 2466-1392(eISSN)
Species characterization of animal by DNA hybridization
DNA hybridization을 이용한 축종특이성 구명
- Lee, Myoung-heon (College of Veterinary Medicine, Chungnam National University) ;
- Kim, Sang-keun (College of Veterinary Medicine, Chungnam National University) ;
- Jung, Gab-soo (National Veterinary Research & Quarantine Service) ;
- Park, Jong-myoung (National Veterinary Research & Quarantine Service)
- Received : 1999.02.01
- Published : 1999.06.22
Abstract
DNA hybridization assay using probes prepared from liver was carried out to identify species characterization of the domestic animals. Gel electrophoresis showed that the target DNA extracted from raw muscle were 1kb and uniform pattern while fragments size of heated muscle were irregular. Hybridization was performed by adding 200ng/ml probe in hybridization solution and incubating for 12 hours at