Korean Journal of Microbiology (미생물학회지)
- Volume 35 Issue 4
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- Pages.302-306
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- 1999
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- 0440-2413(pISSN)
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- 2383-9902(eISSN)
High-density Cultivation and Cryopreservation of Saccharomyces Hansen CBS5926
Saccharomyces cerevisiae Hansen CBS5926의 고농도 배양 및 동결건조 보존
- Bang, Kyu-Ho (Kolon Pharmaceutical Inc. R&D Center) ;
- Kim, Gap-Jin (Kohap Ltd. R&D Institute) ;
- Oh, Deok-Hwan (Kohap Ltd. R&D Institute) ;
- Rhee, Young-Ha (Department of Microbiology, Chungnam National University)
- Published : 1999.01.01
Abstract
Production of biomass by fed-batch culture of Saccharomyces cerevisiae Hansen CBS5926, which is used to treat intestinal disorders, was investigated using ethanol as the sole carbon source. Ethanol was a better carbon source than glucose for high cell density culture of the st-rain since it could decrease the frequency of contamination while increasing the efficiency and final productivity of the fermentation process. Under optimal conditions, 38 g/ℓ of dry cell weight with
정장용 효모로 알려져 있는 Saccharomyces cerevisiae Hansen CBS5926의 생균제 제조를 목적으로 생균수 증대를 위한 배양조건과 배양 후 얻은 균체의 효과적인 동결건조 보존법에 관하여 조사하였다. 포도당을 탄소원으로 하는 회분식과 유가식 배양에 비해 에탄올을 탄소원으로 하는 유가식 배양이 장점이 많은 것으로 나타났으며, 배양 72시간 후 2.2
Keywords
- cryoprotective effect;
- freeze-drying;
- high-density cultivation;
- Saccharomyces cerevisiae Hansen CBS5926