Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지

Molecular Characterization of an Apple cDNA Encoding Cinnamyl Alcohol Dehydrogenase

  • Published : 1999.08.01
Download PDF
⟨ Previous Next ⟩

Abstract

The study of lignin, a major component of secondary cell wall, has been partly focused on its removal from the woody part in the kraft pulping industry. Cinnamyl alcohol dehydrogenase (CAD; EC 1.1.l95) catalyzes the synthesis of cinnamyl alcohols from corresponding cinnamaldehydes. A cDNA clone, MdCADl, encoding putative CAD from apples (Malus domestica Borkh. cv Fuji) was characterized in this study. The clone contains an open reading frame of 325 amino acid residues, which shows a greater than 80％ identity with Eucalyptus CADl. MdCADl mRNA was detectable in vegetative tissues and was strongly expressed in the fruit. The expression pattern of MdCADl mRNA in the fruit peel after light exposure was also examined. The mRNA was rapidly increased until 1 day after light exposure and remained stable thereafter, suggesting that MdCADl is light inducible. The inducibility of the MdCADl gene was examined using several environmental stresses. Mechanical wounding of leaves increased the MdCADl mRNA level and the induction was further increased by salicylic acid. Southern blot hybridization showed that there is either one or a few copies of CAD genes in apples. To our knowledge, it is believed that MdCADl is the first CAD clone expressed predominantly in fruit.

Keywords

References

  1. J. Mol. Biol v.215 Basic local alignment search tool Altschul, S. F.;W. Gish;E. W. Myers;D. Lopman
  2. FEBS Lett v.301 Expansion of the mammalian 3β-hydroxysteroid dehydrogenase/plant dihydroflavonol reductase superfamily to include a bacterial cholesterol dehydrogenase, a bacterial UDP-galactose 4-epimerase, and open reading frames in vaccinia virus and fish lymphocystis disease virus Baker, M. E.;R. Blasco
  3. Plant Physiol v.107 Genomic nucleotide sequence of an Arbidopsis thaliana gene encoding a cinnamyl alcohol dehydrogenase Baucher, M.;J. V. Doorsselaere;J. Gielen;M. V. Montagu;D. Inze;W. Boerjan
  4. Plant Physiol v.112 Red zylem and higher lignin extractability by down-regulating a cinnamyl alcohol dehydrogenase in poplar Baucher, M.;J. V. Doorsselaere;J. Gielen;M. V. Montagu;D. Inze;W. Boerjan
  5. Trends Plant Sci v.3 A new view of lignification Boudet, A.-M.
  6. Basic Methods in Molecular Biology Davis, L. G.;M. D. Dibner;J. F. Batty
  7. Plant Cell v.7 Stress-induced phenylpropanoid metabolism Dixon, R. A.;N. L. Paiva
  8. Trends Plant Sci v.1 Phenylpropanoid matabolism and lignin biosynthesis: From weeds to trees Douglas, C. J.
  9. Anal. Biochem v.132 A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity Feinberg, A.;B. Vogelstein
  10. Plant Mol. Biol v.27 Tissue- and cell-specific expression of a cinnamyl alcohol dehydroegenase promoter in transgenic poplar plants Feuillet, C.;V. Lauvergeat;C. Deswarte;G. Pilate;A. Boudet;J. Grima-Pettenati
  11. Plant Mol. Biol v.23 Molecular cloning, sequence analysis and elicitor-/ozone-induced accumulation of cinnamyl alcohol dehydrogenase from Norway spruce (Piecea abies L.) Galliano, H.;M. Cabane;C. Eckerskorn;F. Lottspeich;H. Jr. Snadermann;D. Ernst
  12. Plant Mol. Biol v.36 A novel aromatic alcohol dehydrogenase in higher plants: Molecular cloning and expression Goffner, D.;J. V. Doorsselaere;N. Yahiaoui;J. Samaj;J. Grima-Pettenati;A. M. Boudet
  13. Eur. J. Biochem v.169 Rapid induction by fungal elicitor of the synthesis of cinnamyl-alcohol dehydrogenase, a specific enzyme of lignin synthesis Grand, C.;F. Sarni;C. J. Lamb
  14. Plant Mol. Biol v.21 Molecular cloning and expression of a Eucalyptus gunnii CDNA clone encoding cinnamyl alcohol dehydrogenase Grima-Pettenati, J.;C. Feuillet;D. Doffner;G. Borderies;A. M. Boudet
  15. Plant J. v.6 Manipulation of lignin quality by down-refulation of cinnamyl alcohol dehydrogenase Halpin, C.;M. E. Knight;G. A. Foxon;M. M. Campbell;A. M. Boudet;J. J. Boon;B. Chabbert;M.-T. Tollier;W. Schuch
  16. Plant Cell Physiol v.34 Cinnamyl alcohol dehydrogenase from Aralia cordata: Cloning of the cDNA and expression of the gene in lignified tissues Hibino, T.;D. Shibata;J.-Q. Chen;T. Higuchi
  17. Plant Cell Physiol v.37 Novel drought-inducible genes in the highly drought-tolerant cowpea: Cloning of cDNAs and analysis of the expression of the corresponding genes Iuchi, S.;K. Yamaguchi-Shinozaki;T. Urao;T. Terao;K. Shinozaki
  18. Plant Mol. Biol v.19 Idenrification and characterization of cDNA clones encoding cinnamyl alcohol dehydrogenase from tobacco Knight, M. E.;C. Halpin;W. Schuch
  19. Plant Physiol v.117 A cDNA encoding cinnamoyl-CoA reductase from Populus trichocarpa (accession no. AJ224986) Leple, J. C.;J. Grima-Pettenati;M. Montagu;W. Boerjan
  20. Mol. Gen. Genet v.247 Genetic analysis of cinnamyl alcohol dehydrogense in loblolly pine: Single gene inheritance, molecular characterization and evoulution MacKay, J. J.;W. Liu;R. Whetten;R. R. Sederoff;D. M. O'Malley
  21. Plant Physiol v.93 Specific inhibition of lignification breaks hypersensitive resistance of wheat to stem rust Moerschbacher, B. M.;U. Noll;L. Gorrichon;H. Reisener
  22. Nucl. Acids Res v.8 Rapid isolation of high molecular weight DNA Murray, M. G.;W. F. Thompson
  23. EMBNEW NEWS v.4 GeneDoc: Analysis and visualization of genetic variation Nicholas, K. B.;H. B. Jr. Nicholas;D. W. Ⅱ.Deerfield
  24. Plant Mol. Biol v.38 Cloning and characterization of two maize cDNAs encoding cinnamoyl Co-A reductase (CCR) and differential expression of the corresponding genes Pichon, M.;I. Courbou;M. Beckert;A. M. Boudet;J. Grima-Pettenati
  25. Science v.277 Abnormal lignin in a loblolly pine mutant Ralph, J.;J. J. Mackay;R. D. Hatfield;D. M. OMalley;R. W. Whetten;R. R. Sederoff
  26. Plant Molecular Biology Manual Extraction of DNA from plant tissues Rogers, S. O.;A. J. Bendich;S. B. Gelvin;R. A. Schilperoort;D. P. S. Verma(eds.)
  27. Handbook of Fruit Science and Technology: Production, composition, storage, and processing Salumkhe, D. K.;S. S. Kadam
  28. Molecular Cloning: A Laboratory Manual (2nd ed) Sambrook, J.;E. F. Fritsch;T. Maniatis
  29. Proc. Natl. Acad. Sci. USA v.74 DNA sequencing with chain terminating inhibitors Sanger, F.;S. Nicklen;A. R. Coulson
  30. Lignins Occurrence, formation, structure and reactions Sarkanen, K. V.;H. L. Hergert;Sarkanen, K. V.;C. H. Ludwig(eds.)
  31. Plant Physiol v.113 Changes in the activity and mRNA of cinnamyl alcohol dehydrogenase during tracheary element differentiation in zinnia Sato, Y.;T. Watanabe;A. Komamine;T. Hibino;D. Shibata;M. Sugiyama;H. Fukuda
  32. Mol. Cells v.5 Expressed sequence tags of fruits, peels, and carpels and analysis of mRNA expression levels of the tagged cDNAs of fruits from the Fuji apple Sung, S.-K.;D.-H. Jung;J. Nam;S.-H. Kim;S.-R. Kim;G. An
  33. Annu. Rev. Plant Physiol. Plant Mol. Biol v.46 Light-regulated transcription Terzaghi, W. B.;A. R. Cashmorc
  34. Nucl. Acids Res v.22 CLUSTAL W:Improving the sensitivity of progressive mutiple sequence alignment through sequence weighting, positions-specific gap penalties and weight matrix choice Thompson, J. D.;D. G. Higgins;T. J. Gibson
  35. Plant Cell Physiol v.36 Molecular cloning and characterization of Rosa hybrida dihydroflavonol 4-reductase gene Tanaka, Y.;Y. Funkui;M. Fukuchi-Mizutani;T. A. Holton;E. Higins;T. Kusumi
  36. Plant Cell v.4 Acquired resistance in Arabidopsis Uknes, S.;B. Mauch-Mani;M. Moyer;S. Potter;S. Williams;S. Dincher;D. Chandler;A. Slusarenko;E, Ward;J. Ryals
  37. Proc. Natl. Acad. Sci. USA v.85 Cinnamyl-alcohol dehydrogenase, a mulecular marker specific for lignin synthesis: cDNA cloning and mRNA induction by fungal elicitor Walter, M. H.;J. Grima-Pettenati;C. Grand;A. M. Boudet;C. J. Lamb
  38. Plant Physiol v.103 Pathway of salicylic acid biosynthesis in healthy and virus-inoculated tobacco Yalpani, N.;J. Leon;M. Lawton;I. Raskin