Korean Journal of Plant Tissue Culture (식물조직배양학회지)

The Korean Society of Plant Biotechnology (한국식물생명공학회)
권호 표지

Resistance to the Fungal Pathogen Phytophthora infestans of Transgenic Potato Plants Harboring of Chitinase Gene

Chitinase 유전자 도입 형질전환 감자식물체의 역병저항성

  • 최경화 (생명공학연구소 식물조직배양 R.U.) ;
  • 양덕춘 (한국인삼연초연구원 유전생리부) ;
  • 김현순 (생명공학연구소 식물조직배양 R.U.) ;
  • 최경자 (한국화학연구소 농약활성연구실) ;
  • 조광연 (한국화학연구소 농약활성연구실) ;
  • 정혁 (생명공학연구소 식물조직배양 R.U.)
  • Published : 1999.07.01
Download PDF
⟨ Previous Next ⟩

Abstract

A fungal infection assay between normal and transgenic potato harboring chitinase gene in cultivar Belchip was investigated. In the first stage of experiment, seven transgenic lines having 12cm tall were tested for their resistance against potato late blight pathogen Phytophthora infestans by infection with the zoospores, artificially, Susceptibility to potato late blight infection could be classified into three types based on the rate. In terms of resistance to the disease, two lines were higher, two lines were more suppressive, and three lines were similar as compared with the control. In the following experiment, only 2 risistant lines and 1 suppressed line were used to confirm the resistance again. The results of both experiments were similar. Furthermore, two highly resistant transgenic lines grown in field exhibited a higher resistance than control under the conditions of natural ocurrence of the fungal disease.

Chitinase 유전자가 삽입된 감자 Belchip 품종의 형질전환체와 대조구에 곰팡이 병원균을 접종하였다. 7개 계통의 형질전환식물체를 12cm정도 키운 후 병원균인 Phytophthora infestans의 zoospore를 접종하여 인공적으로 역병을 유발시켰다. 그 결과 발병율에 따라서 세 그룹으로 분리되었는데 대조구에 비하여 감염 정도가 심한 것 2개 계통. 비슷한 3개 계통, 발병 정도가 약한 2개 계통으로 구분되었다. 대조구에 비하여 저항성이 높았던 2개 계통과 발병이 심했던 1개 계통만을 대상으로 하여 2차실험을 실시한 결과, 1차실험과 비슷한 경향을 나타냈다. 포장에서 생육된 2개의 저항성 계통은 자연적으로 발생한 역병에 대해서도 역시 대조구에 비하여 역병저항성이 더 높았다.

Keywords

References

  1. Seattle Systemic acquired resistance in tobacco: use of transgenic expression to study the functions od pathogenesis-related proteins. Abstr. 70, Program and Abstracts. Sixth International Symposium on Molecular Plant Microbe interactions Alwxander, D.;Giascock, C.;Pear, J.;Stinson, J.;Ahi-Goy, P.;Gut-Rella, M.;Ward, E.;Goodman, R.;Ryals, J.
  2. Science v.254 Transgenic plants with enhanced resistance to the fungal pathogen Rhizoctonia solani Broglie, K.;Chet, I.;Holliday, M.;Cressman, R.;Biddle, P.;Knowlton, S.;Mauvais, C. J.;broglie, R.
  3. J. Biotech Expression of Chitinase Gene in Solanum tubersum L. Choi, K. H.;Yang, D. C.;Jeon, J. H.;Kim, H. S.;Joung, Y. H.;Joung, H.
  4. Am. Phytopatol Soc. v.73 Occuttence of metalaxyl-resistant isolates of Phytophthora iunfestans in potato fields in lstael Cohen, Y.;Reuveni, M.
  5. Euphytoca Suppl Resistance to late blitht(Phytophthora infestans) in ten wild Solanum species Colon, L. T.;Budding, D. I.
  6. Transgenic Res. v.7 Insect resistance of transgenic tobacco expressing an insect chitinase gene Ding, X.;Gopalakrishnan, B.;Johnson, L. B.;White, F. F.;Wang, X.;Muthukrishnan, S.
  7. Biol. Tech. v.12 Transgene inactivayion : Plant fight back Finnegan, J.;Mcelory, D.
  8. The Plant J. v.8 Enhanced quantitative resistance against fungal sidease by combinatorial expression of different varlwy antifungal proteins in transgenic tobacco Jach, G.;Gornhardt, B.;Mundy, J.;Logemann, J.;Pinsdorf, E.;Leah, R.;Scheel, J.;Maas, C.
  9. Ministry Sci. Tech. v.MAN81460-756-4 Study on the development of mass production technology for the transgenic seed pototo Joung, H.
  10. Biotechnology v.10 Emerging stratrgies foe enhancing crop resistance to microbial pathodens Lamb, C. J.;Ryals, J. A.;Ward, E. R.;Dixon, R. A.
  11. Proc. Natl. Acad. Sci. v.91 Osmotin overexpression in potato delays development of disease sumptoms Liu, D.;Raghorhana, K. G.;Hasegawa, P. M.;Bressan, R. A.
  12. Proc. Natl. Acad. Sci. v.95 Genes from mycoparasitic fungi as a source for improving plant resistance to fungal pathofens Lorito, M.;Woo, S. L.;Eernandez, I. G.;Colucci, G.;Harman, G. E.;PintorToro J, A.;Filippone, E.;Muccifora, S.;Lawrence, C. B.Zoina, A.;Tuzun, S.;Scala, F.
  13. The EMBo J. v.8 A phenylalanine ammonia-lyase gene from parsely: structure, regulation and identification of elicitor and light respinsive cis-acting elements Lois, R.;Schulz, W.
  14. Plant Mol. Biol. v.16 High level expression of a tobaco chitinase gene in Nicotiana nicotianae infection Neuhas, J. M.;Ahi-Goy, P.;Hinz, U.;Flores, S.;Meins, F.
  15. Plant J. v.2 The methylation pattem of chromosomal integration regions gene activity of transgerred DNA in Petunia hybrida Prols, F.;Meyer, P.
  16. Plant Mol. Biol. v.25 Effect of chitinase antisense RNA expression on disease susceptibility of Arabidopsis plants. Samac, D. A.;Shah, D. M.
  17. Mol. Gener v.202 Structure of the chalcone synthase gene of Antirrhunum majus Sommer, H.;Saedler, H.
  18. Proc. Natl. Acad. Sci. v.83 Rapid activation by fungal elicitor of genes encoding "pathofenesisrelated" proteins in culture parsley cells Somssich, I. E.;Schmelzer, E.;Bollmann, J.;Hahlbrock, K.
  19. Plant. Cell. Rep. v.17 Transgenic cucumber plants harboring a rice chitinase gene exhibit enhanced resistance to gray mold (Botrytis cinerea) Tabei, Y.;Kitade, S.;Nishizawa, Y.;Kikuchi, N.;Kayano, T.;Hivi, T.;Akutsu, K.
  20. Plant Cell. Rep. v.16 A fungal chitinase gene from Rhizopus oligosporus confers antifungal activity to transgenic tobacco Terakawa, T.;Takaya, N.;Horiuchi, H.;Koike, M.
  21. Am. Phytopath Soc. St. Paul. Control of Phytopthora by host redidtance: problems and progress Umaerus, V.;Umaerus, M.;Erjfalt, L.;Nilsson, B.A.;Erwin, D. C.(ed.);Bartnicki-Garcia, S.(ed.);Rsao, P. H.(ed.)
  22. Plant Physiol. v.91 Avscisic acid suppression of phenylalanine ammonia-lyase acrivity and mRNA and resistance of soybeans to Phytopthor megasperma Ward, E. W. B.;Cahill, D.;Bhattacharyya, M. K.
  23. Adv. Plant Pathol. v.7 Greeding for resistance Wastie, R. L.
  24. Mol. Gen. Genet. v.203 Molecular cloning of the c2 locus of Zea mays, the gene coding for chalcone synthase Wienand, U.;Wqydemann, U.;Niesbach-Klosgen, U.;Peterson, P. A.;Saedler, H.
  25. Planta v.198 Analysis of late-blight disease resistance and freezing tolerance in transgenic potato plants expressing sense and antisense genes for an osmotin like protein Zhu, B.;Chen, T. H. H.;Li, P. H.