Journal of Aquaculture (한국양식학회지)

The Korean Society of Fisheries and Aquatic Science (한국양식학회)
권호 표지

Gold Storage and Cryopreservation of Grey Mullet(Mugil cephalus) Sperm

숭어(Mugil cephalus) 정자의 냉장.냉동보존

  • Published : 1999.03.01
Download PDF
⟨ Previous Next ⟩

Abstract

Experiments were performed to study the activity and fertility of grey mullet (Mugil cephalus) sperm after the courses of cold storage and cryopreservation. The head of spermatozoon showing spherical shape was sized $1.26{\pm}0.08 \{mu}textrm{m}$ in diameter and its nucleus contained numerous granular chromatins. Flagellum of tail showed typical 9＋2 structure. Preservation of grey mullet sperm was the most effective when it was stored with serum of the same species at $0^{\circ}C$ and sperm activity index was similar in egg-tris, 0.1 M, 0.3 M and 0.5 M glucose. When grey mullet sperm were cryopreserved in MFRS as diluent with 10% dimethyl sulfoxide was effective compared with other diluents. Some of post-thawed spermatozoa showed the enlarged head and ruptured plasma membrane compared with unfrozen spermatozoa.

숭어 정액의 냉장 및 냉동보존시 적합한 희석액을 선정하고, 알에 대한 수정률을 평가하였다. 비보존 숭어 정자의 머리는 공모양으로 직경 $1.26{\pm}0.08 \{mu}textrm{m}$, 길이 $1.06{\pm}0.07 \{mu}textrm{m}$ 였으며, 과립상의 염색질을 가지고 있었다. 숭어 정자의 냉장보존시($0^{\circ}C$, 10일간) 희석액으로는 동종의 혈정이 가장 높은 정자활성을 나타냈으며, egg-tris, 0.1M, 0.3M 및 0.5M glucos에서는 활성이 서로 비슷하였다. 또한 동해방지제로 10% DMSO, 희석액으로 MFRS를 사용하여 냉동보존한 후 해동시켰을 때 대조구와 유사한 수정률을 보였다. 냉동보존 후 해동시킨 일부 정자 중에서는 세포막이 이탈되거나 소실되는 구조적 변화를 나타냈다.

Keywords

References

  1. Nature v.172 Sperm storage and cross-fertilization of spring and autumn spawning herring Blaxter, J. H. S.
  2. Crybiology v.37 Sublethal damage during cryopreservation of rainbow trout sperm Cabrita E.;R. Alvarez;L. Anel;K.J. Rana;M. P.Herraez
  3. Sparus aurata. Aquaculture v.90 A diluent for sperm cryopreservation of gilthead seabream Chambeyron, F.;Y. Zohar
  4. Aquaculture v.5 Study on cryogenic preservation of grey mullet sperm Chao, N. H.;H. P. Chen;I. C. Liao
  5. Epinephelus malabaricus (Bloch and Schneider) Asian Fish. Sci. v.5 Short- and long-tern cryopreservation of sperm and sperm suspension of the grouper Chao, N. H.;H. P. Tsai;I. C. Liao
  6. Porgr. Fish-Cult. v.55 Cryopreservation of yellow perch semen Ciereszko, A.;L. Ramaseyer;K. Dabrowski
  7. Annales des sciences naturelles : Troisieme serie v.19 Recherches sur lavitalire des spermotozoides de quelques poissons d'eau douce De Quatrafages, M.A.
  8. Cryobiology v.37 Effect of cryopreservation on the fine structure of spermatozoa of rainbow trout (Oncorhynchus mykiss) and brown trout (Salmotrutta F. fario) Drokin, S.;H. Stein;H. Bartsherer
  9. Aquaculture v.43 Some factors affecting the preservation of salmon spermatozoa Erdahl, A. W.;D. A. Erdahl;E. F. Graham
  10. Trans. Am. Fish. Soc. v.3 Preservation of channel catfish sperm Guest, W. C.;J. W. Avault;J. D. Roussel
  11. Theriogenology v.41 Cryopreservation of yellowfin seabream (Acathopagrus latus) spermatozoa (teleost, perciformes, sparidae) Gwo, J. C.
  12. J. Exp. Zool. v.264 Cryopreservation of Atlantic croaker spematozoa : Evaluation of morphological changes Gwo. J. C.;C. R. Arnold
  13. Aquaculture v.94 Cryopreservation of Atlantic croaker spermatozoa Gwo, J. C.;K. Strawn;M. T. Longnecker;R. Connie
  14. Aquaculture v.28 A comparative study of various extenders for milk fish Chanos chanos (Forsskal) sperm preservation Hara, S.;J. T. Canto;M. E. Almendras
  15. Aquaculture v.32 Cryopreservation of Sarotgerodon mossambicus spermatoza Harvey, B.
  16. Aquaculture v.142 Diluent for cool storage of Japanese eel (Anguilla japonica) spermatozoa Ohta, H.;T. Izawa
  17. Aquaculture v.32 Cryopreservation of rainbow trout (Salmo gairdneri) sperm.IV. The effect of DMSO concentration and equilibration time on sperm survival, sucrose and KCI as extender components and the osmolality of the thawing solution Stoss, J.;W. Holtz
  18. J. Fish. Res. Bd. Can. v.26 An improved extender for freezing Atlantic salmon spermatozoa Truscott, B.;D. R. Idler
  19. Aquaculture v.27 Preliminary observation of chilled and deep-frozen storage of grouper (Epinephelus tauvina) sperm Withtler, F. C.;L. C. Lim
  20. Aquaculture v.102 Cryopreservation of summer whiting (sillago ciliata) spermatozoa Young, J. A.;M. F. Capra;A. W. Blackshaw
  21. 농림수산특정연구사업 최종연구보고서 해산어류 정자의 생리활성과 장·단기 보전 장영진;강용진;김승헌;임한규;이정용;강덕영;고강희;장윤정;최윤희
  22. 精子學 森澤正昭;星元紀