KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Characterization and Modeling of Growth Properties of Petroleum Desulfurizing Bacterium Desulfovibrio sp. B5

석유탈황 미생물 Desulfovibrio sp. B5의 생육특성과 성장 Modeling

  • 신철수 (서울대학교 식품공학과 및 농업생물신소재 연구센터) ;
  • 김명동 (서울대학교 식품공학과 및 농업생물신소재 연구센터) ;
  • 안장우 (청강문화산업전문대학 식품공학과) ;
  • 신평균 (한국과학기술원 환경연구부) ;
  • 서진호 (서울대학교 식품공학과 및 농업생물신소재 연구센터)
  • Published : 1999.02.01
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Abstract

This study was focused on investigating the growth properties of a sulfate reducing bacterium Deslfovibrio sp. B5 which has metabolic ability for desulfurization of petroleum. The optimal temperature and pH for growth of Desulfovibiro sp. B5 were $38^{\circ}C$ and 6.6-7.0, respectively. Addition of 10% corn steep liquor to the Postgate medium C resulted in 0.79 g/L cell concentration, corresponding to a 1.8-fold increase in dry cell mass. Acetate concentrations above 10g/$\ell$ inhibited cell growth significantly. $H_2S$ generated from the sulfate reduction also inhibited the growth of Desulfovibrio sp. B5 at a concentration of 10mM total sulfide. But $N_2$ gassing relieved the growth inhibition by $H_2$S and thereby resulted in a 1.75-fold enhancement in specific growth and lactate consumption pattern of Desulfovibrio sp. B5.

본 연구에서는 석유의 탈황에 이용될 수 있는 미생물인 Desuljovrvbrio sp. B5의 생육특성을 조사하였다. Postgate medium C에서의 생육 최적 온도는 $38^{\circ}C$,pH는 6.6~7.0으로 나타났다. Corn steep liquer를 대체 배지성분으로 사용한 결과 0.79g/L의 균체를 얻을 수 있었으며 이는 기존의 결과와 비교하여 약 1.8배 증가한 값이다. Postgate medium C에서 Lactate의 대사 과정에서 생성되는 acctate는 10g/L 이상에서 성장저해를 유발하였다. $H_2S$는 10mM의 total sulfide 농도에서도 균체성장이 저해되었지만 이는 질소가스 주입으로 해소할 수 있었다. 또한 회분식 배양의 결과와 $H_2S$에 의한 product ingixtion에 근거한 균체성장의 modeling 결과는 잘 일치하였다.

Keywords

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